Human PTEN Knockout Cell Line-Panc-1
Cat.No. : CSC-RT2664
Host Cell: Panc-1 Target Gene: PTEN
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2664 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human PTEN using CRISPR/Cas9. |
| Target Gene | PTEN |
| Host Cell | Panc-1 |
| Size Form | 1 vial (10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid nirtogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
PTEN (phosphatase and tensin homolog) is an important tumor suppressor gene that plays an important role in regulating the cell cycle and preventing cells from growing and dividing too rapidly or uncontrollably. PTEN was first discovered in 1997 and is located on chromosome 10 (10q23), and is known for encoding a protein with dual-specificity phosphatase activity. This activity enables it to remove phosphate groups from proteins and lipids, specifically targeting the lipid second messenger phosphatidylinositol (3,4,5)-triphosphate (PIP3), thereby acting as a negative regulator of the PI3K/AKT signaling pathway.
PTEN gene mutations or deletions are commonly found in various types of cancer, including breast cancer, prostate cancer, endometrial cancer, glioblastoma, etc. Loss of PTEN function can lead to uncontrolled cell growth and survival, significantly promoting tumor development and progression. Furthermore, germline mutations in the PTEN gene have been associated with several inherited disorders, collectively known as PTEN hamartoma syndrome (PHTS), including Cowden syndrome, Bannayan-Riley-Ruvalcaba syndrome, and Proteus syndrome. In addition to its known role in cancer suppression, recent studies have uncovered broader effects of PTEN in other biological processes, such as metabolism, aging, and neurodevelopment.
The Human PTEN Knockout Cell Line (Panc-1) is a crucial tool in biomedical research, primarily used to study the role of PTEN (Phosphatase and Tensin Homolog) in cancer biology, especially in pancreatic cancer. Here are some key applications:
Cancer Research: The PTEN Knockout Cell Line-Panc-1 is extensively used in cancer research to study the role of the PTEN gene in tumorigenesis. PTEN is a well-known tumor suppressor, and its knockout in Panc-1 cells helps investigate the molecular mechanisms of pancreatic cancer progression and resistance to therapy.
Drug Testing: This cell line is instrumental in drug discovery and development. By using PTEN-deficient Panc-1 cells, researchers can screen potential therapeutic compounds that could target the pathways altered due to loss of PTEN, potentially leading to new cancer treatments.
Signal Transduction Studies: PTEN is a critical regulator of the PI3K/AKT signaling pathway. Researchers utilize the PTEN Knockout Cell Line-Panc-1 to explore alterations in cell signaling events, aiding in the understanding of how PTEN loss affects various cellular processes, including growth, survival, and metabolism.
Genetic Research: This cell line serves as a valuable model for genetic studies focused on gene function and interaction. It provides insights into the consequences of PTEN deletion at a genetic level, contributing to broader knowledge about gene networks and regulatory mechanisms in human cells.
For research use only. Not intended for any clinical use.
