Human PKD2 Knockout Cell Line-HEK293
Cat.No. : CSC-RT2611
Host Cell: HEK293 Target Gene: PKD2
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
Inquire for Price
Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2611 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human PKD2 using CRISPR/Cas9. |
| Target Gene | PKD2 |
| Host Cell | HEK293 |
| Size Form | 1 vial (10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid nirtogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Inquiry
Background
Applications
The PKD2 gene, also known as polycystic kidney disease 2, is an important gene that plays a key role in human physiology and pathology. The PKD2 gene is located on chromosome 4q22.1 and encodes a membrane protein called polycystin-2, which is involved in the formation and function of primary cilia and calcium signaling pathways. The main role of the PKD2 gene is closely related to its function in calcium ion transport and its interaction with other proteins such as polycystin-1 encoded by the PKD1 gene. Both polycystin-1 and polycystin-2 are essential for maintaining the structure and function of the renal tubules.
PKD2 gene mutations are associated with autosomal dominant polycystic kidney disease (ADPKD), a common inherited disease characterized by the presence of numerous cysts in the kidneys. The disease can lead to progressive renal failure, hypertension, and other systemic complications. Although PKD2 mutations cause a smaller proportion of ADPKD cases compared to PKD1 mutations, the clinical manifestations of PKD2-related ADPKD tend to be milder and occur later. In addition to its role in ADPKD, studies suggest that the PKD2 gene may have other physiological functions and may be involved in other diseases involving ciliary function and calcium signaling.
PKD2 encodes polycystin-2, a key protein involved in kidney function and cellular processes. Creation of a PKD2 knockout model in HEK293 cells has helped to better understand the molecular mechanisms behind PKD and aid in drug discovery, gene function studies, and therapeutic development. Below are the main applications of the human PKD2 knockout cell line - HEK293.
Polycystic Kidney Disease (PKD) Research: PKD2 knockout HEK293 cells provide a simplified in vitro model to study the pathophysiology of polycystic kidney disease. By examining the cellular and molecular consequences of PKD2 deficiency, researchers can identify disease mechanisms, progression, and key regulatory pathways.
Functional Analysis of Polycystin-2: Loss of PKD2 in HEK293 cells highlights the role of polycystin-2 in cellular processes such as calcium signaling, cell proliferation, and apoptosis. This helps to study how polycystin-2 interacts with other proteins and regulates cellular homeostasis.
Drug Screening and Development: Using PKD2 knockout HEK293 cells, researchers can screen for potential therapeutic compounds that can compensate for loss of polycystin-2 function or correct its downstream effects. This cell line provides a high-throughput system to test drug efficacy and toxicity, thereby accelerating the identification of promising drug candidates.
Molecular and Cellular Pathway Elucidation: By comparing PKD2 knockout cells to wild-type HEK293 cells, researchers can identify alterations in cellular signaling pathways, gene expression profiles, and metabolic processes. This comparative analysis helps delineate pathways affected by PKD2 loss and may reveal new therapeutic targets.
For research use only. Not intended for any clinical use.
