Human PITPNA Knockout Cell Line-HEK293
Cat.No. : CSC-RT2763
Host Cell: HEK293 Target Gene: PITPNA
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2763 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human PITPNA using CRISPR/Cas9. |
| Target Gene | PITPNA |
| Host Cell | HEK293 |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid Nitrogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The PITPNA gene, also known as phosphatidylinositol transfer protein Alpha, is a member of the phosphatidylinositol transfer protein (PITP) family and plays a crucial role in lipid signaling and metabolism. The PITPNA gene is located on chromosome 17p13.3 in humans and chromosome 11 B5 in mice. The protein encoded by PITPNA is expressed in various tissues, with higher levels in the retinal pigment epithelium, parotid gland, pons, and specific regions of the skin and brain. One of the main functions of the PITPNA protein is to bind and transfer phosphatidylinositol (PI) and phosphatidylcholine (PC) between cell membrane compartments. This transport process is essential for the synthesis of phosphoinositides, which are key signaling molecules that regulate various cellular activities, including cell growth, differentiation, and survival.
Research has shown that the PITPNA gene plays an important role in neural development and function. Mutations or defects in this gene have been associated with a variety of neurological diseases. For example, alterations in PITPNA expression can affect synaptic function and plasticity, which can lead to cognitive impairment and neurodegenerative diseases. In addition to its neurological importance, PITPNA has been implicated in other physiological processes. The gene has been observed to be involved in regulating insulin secretion from pancreatic beta cells, highlighting its potential link to metabolic disorders such as diabetes.
Functional Analysis of PITPNA: Knockout of the PITPNA gene in HEK293 cells allows researchers to study the specific functions and mechanisms of the PITPNA protein in cellular processes. By comparing knockout cells to wild-type cells, scientists can elucidate the role of PITPNA in phospholipid metabolism, signal transduction, and intracellular trafficking.
Disease Modeling and Drug Discovery: PITPNA has been implicated in a variety of human diseases, including neurological disorders and cancer. The HEK293 PITPNA knockout cell line serves as an in vitro disease model, providing a valuable platform for identifying and testing new therapeutics and understanding the molecular basis of these diseases.
Pathway Dissection and Mechanistic Studies: Researchers can dissect the involvement of PITPNA in specific pathways by examining changes in cellular responses, protein interactions, and pathway activation.
Genetic Interaction Studies: The HEK293 PITPNA knockout cell line can be used in conjunction with other genetic manipulations to study epistatic relationships and reveal genetic interactions.
Validation of Antibodies and Assays: The PITPNA knockout cell line can serve as a negative control in the validation of antibodies, assays, and other experimental tools. This ensures that the tools are specific to PITPNA and helps avoid cross-reactivity and nonspecific binding in the experimental setup.
For research use only. Not intended for any clinical use.
