Human PIP5K1C Knockout Cell Line-HEK293
Cat.No. : CSC-RT2765
Host Cell: HEK293 Target Gene: PIP5K1C
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2765 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human PIP5K1C using CRISPR/Cas9. |
| Target Gene | PIP5K1C |
| Host Cell | HEK293 |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid Nitrogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The PIP5K1C gene, also known as phosphatidylinositol-4-phosphate 5-kinase type 1 gamma, encodes an enzyme that plays an essential role in cellular processes in Homo sapiens (human species). It is a member of the type I phosphatidylinositol-4-phosphate 5-kinase family and is essential for the production of phosphatidylinositol-4,5-bisphosphate (PIP2), a key phospholipid involved in various cellular mechanisms such as signal transduction, cytoskeletal arrangement, and membrane trafficking. The enzyme is able to bind to the FERM domain of talin through its C-terminus, which emphasizes its role in the dynamic regulation of the actin cytoskeleton and the stabilization of integrin-mediated cell adhesion.
In the cellular context, PIP5K1C exhibits multiple functions, including promoting endocytosis, regulating synaptic vesicle efflux, and managing cell-cell adhesion. Its influence extends to the assembly of adherens junctions, the organization of the actin cytoskeleton, and the formation of membrane ruffles, thus emphasizing its potential impact on cell morphology and motility. Furthermore, the enzymatic role of PIP5K1C and its involvement in clathrin-mediated endocytosis underscore its importance in vesicle trafficking processes. Its expression is prominent in tissues such as the right hemisphere of the cerebellum, testis, and endometrial stromal cells, highlighting its diverse expression pattern depending on the cellular functions required in different tissues.
Human PIP5K1C knockout cell line - HEK293 is a valuable tool for biological and medical research. The following are the main applications of this cell:
1. Signal Transduction Studies: These cell lines are essential for studying the role of PIP5K1C in signal transduction pathways. By analyzing the effects of PIP5K1C knockout on signaling dynamics, researchers can elucidate the molecular pathways in which PIP5K1C participates and how its absence affects cellular communication and responses.
2. Membrane Trafficking Analysis: PIP5K1C is involved in the regulation of membrane trafficking processes. Knockout cell lines allow detailed studies of how PIP5K1C loss affects endocytosis, exocytosis, and vesicle trafficking, which is essential for understanding cellular logistics and function.
3. Phosphatidylinositol Studies: Since PIP5K1C is a key enzyme in the phosphatidylinositol signaling pathway, its knockout facilitates the study of phosphatidylinositol-dependent pathways. Researchers can better understand the role of this enzyme in phosphatidylinositol turnover and its broader impact on cell physiology.
4. Cancer Research: PIP5K1C knockout cell lines can be used to study its role in tumorigenesis and cancer progression. Given that PIP5K1C is associated with cell proliferation and survival, its knockout allows researchers to explore the possibility of novel cancer treatments targeting this enzyme.
5. Drug Development and Screening: PIP5K1C knockout cell lines can be used to screen potential drugs to understand their efficacy in the absence of this enzyme. This application is critical to identifying drugs that can modulate pathways and processes that depend on PIP5K1C, thereby aiding drug discovery and development.
For research use only. Not intended for any clinical use.
