Human OGG1 Knockout Cell Line-HEK293T
Cat.No. : CSC-RT2740
Host Cell: HEK293T Target Gene: OGG1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
Inquire for Price
Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2740 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human OGG1 using CRISPR/Cas9. |
| Target Gene | OGG1 |
| Host Cell | HEK293T |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid Nitrogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Inquiry
Background
Applications
OGG1, also known as oxoguanine glycosylase, is a key gene that encodes the enzyme responsible for excising 8-oxoguanine (8-oxoG) from DNA. 8-oxoG is a mutagenic base byproduct generated by exposure to reactive oxygen species (ROS) that, if not repaired, can lead to mutations. OGG1 plays an important role in the base excision repair pathway, a key mechanism for maintaining genomic integrity in a variety of organisms, including humans, bacteria, archaea, and other eukaryotes.
The activity of OGG1 is regulated by oxidative stress. Under stress, OGG1 may be temporarily inactivated, which results in increased recruitment of transcription factors such as NFkB, which stimulates the expression of inflammatory genes. This suggests that OGG1 plays a role not only in genome maintenance, but also in the regulation of inflammatory responses. Studies have shown that mice lacking OGG1 have increased levels of 8-oxo-dG in both nuclear and mitochondrial DNA. These mice are also more susceptible to obesity, insulin resistance, and a higher incidence of cancer, especially when the MTH1 gene is also disrupted.
Cancer Research: The Human OGG1 Knockout Cell Line - HEK293T is widely used in cancer research to study the role of oxidative DNA damage in cancer development. By knocking out the OGG1 gene, researchers can study how the loss of OGG1 affects cell proliferation, mutation rates, and overall cancer progression.
DNA Repair Mechanisms: This cell line is essential for studying DNA repair mechanisms, specifically base excision repair (BER). OGG1 is a key enzyme in the BER pathway, responsible for repairing 8-oxoguanine damage caused by oxidative stress. By using a knockout version of OGG1, scientists can better understand how cells compensate for the loss of this repair enzyme and identify other proteins and pathways involved in maintaining genomic integrity.
Neurodegenerative Disease Research: The OGG1 gene has been implicated in neurodegenerative diseases such as Alzheimer's and Parkinson's diseases. The Human OGG1 Knockout Cell Line - HEK293T provides a model to study how oxidative DNA damage contributes to neuronal cell death and the progression of neurodegenerative diseases. This could help identify potential biomarkers and therapeutic targets for these diseases.
Drug Screening: This cell line can be used in high-throughput drug screening assays to identify compounds that modulate oxidative DNA damage and repair. By comparing the effects of candidate drugs on OGG1 knockout cells and wild-type cells, researchers can identify drugs that enhance or inhibit the DNA repair process, thereby aiding the development of new drugs for the treatment of various diseases associated with oxidative stress.
Aging Research: Oxidative DNA damage is a major factor in the aging process. The human OGG1 knockout cell line - HEK293T allows resea rchers to study how OGG1 loss affects cellular aging.
For research use only. Not intended for any clinical use.
