Human NYNRIN Knockout Cell Line-PC3
Cat.No. : CSC-RT2783
Host Cell: PC3 Target Gene: NYNRIN
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
Inquire for Price
Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2783 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of NYNRIN using CRISPR/Cas9. |
| Target Gene | NYNRIN |
| Host Cell | PC3 |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid Nitrogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Inquiry
Background
Applications
NYNRIN (NYN domain and retroviral integrase) is a gene that may have played an important role in the evolutionary emergence of placental structures in therian mammals. NYNRIN is derived from the fusion of a gene containing KH and NYN domains (KHNYN) with fragments from the Ty3/Gypsy retrotransposon, including the reverse transcriptase ribonuclease H (RNase H) domain and the integrase (INT) domain. The gene appeared in the therian lineage, a critical period marked by the emergence of the placenta, which contributed to the evolutionary success of therian mammals.
NYNRIN expression was found to be consistently elevated in placental tissues of therian mammals, with some exceptions, such as the Tamal kangaroo, which may be attributed to gaps in the data or the ambiguous invasive nature of the placental type in this species. Functional analysis using siRNA specifically targeting NYNRIN in a human trophoblast cell line revealed its key role in trophoblast invasion. Knockdown of NYNRIN significantly inhibited the invasive ability of HTR8/SVneo cells, while this behavior was not observed with knockdown of its non-retroviral-derived homolog KHNYN, suggesting that there was a significant evolutionary functional divergence after the gene fusion, with NYNRIN acquiring and refining a role that is indispensable for placental function.
Applications of Human NYNRIN Knockout Cell Line-PC3
1. Cancer Research: Human NYNRIN Knockout Cell Line-PC3 is widely used in cancer research, especially for studying prostate cancer. By knocking out the NYNRIN gene, researchers can better understand the role of this gene in prostate cancer progression and metastasis.
2. Gene Function Analysis: Researchers use it to observe changes in cell behavior, gene expression, and protein interactions due to the loss of NYNRIN, thereby gaining insight into its regulatory mechanisms.
3. Drug Discovery and Development: NYNRIN Knockout Cell Line-PC3 can be used to screen and develop new therapeutic drugs. This cell line helps to identify potential drug targets and evaluate the efficacy and toxicity of new compounds in preclinical studies.
4. Signal Transduction Studies: This cell line can be used to study signal transduction pathways affected by the NYNRIN gene. By observing changes in the signaling cascade, researchers can decipher the pathways that NYNRIN may affect and how its knockout changes the response of cells to various stimuli.
5. Apoptosis and Cell Cycle Studies: It was also used to study the effects of NYNRIN knockout on apoptosis (programmed cell death) and cell cycle regulation.
For research use only. Not intended for any clinical use.
