Human MC4R Knockout Cell Line-HEK293T
Cat.No. : CSC-RT2778
Host Cell: HEK293T Target Gene: MC4R
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2778 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human MC4R using CRISPR/Cas9. |
| Target Gene | MC4R |
| Host Cell | HEK293T |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid Nitrogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The melanocortin 4 receptor (MC4R) gene is located on human chromosome 18 and encodes the MC4R protein, a G protein-coupled receptor that is primarily expressed in the brain, particularly in regions such as the hypothalamus that are involved in appetite and energy balance. MC4R is a key component of the central melanocortin pathway, influencing energy expenditure and food intake. Activation of MC4R by its natural ligands, such as α-melanocyte stimulating hormone (α-MSH), results in decreased food intake and increased energy expenditure, thus promoting weight loss. Conversely, inhibition or dysfunction of MC4R leads to overeating (excessive eating) and decreased energy expenditure, resulting in obesity.
MC4R gene mutations are one of the most common genetic causes of severe obesity. Both heterozygous and homozygous mutations have been found, with the latter generally resulting in a more severe phenotype. These mutations disrupt the normal function of the receptor, resulting in impaired melanocortin pathway signaling. As a result, individuals with MC4R dysfunction may be more susceptible to weight gain and may find weight control more challenging. In addition to its role in obesity, MC4R is involved in other physiological processes, including regulation of glucose metabolism, sexual function, and cardiovascular maintenance. Studies have shown that MC4R mutations may also be associated with other metabolic disorders, such as insulin resistance and type 2 diabetes.
The human MC4R knockout cell line - HEK293T is a valuable tool in scientific research and drug development. The following are the main applications of this cell line:
Obesity and Metabolic Disorder Research: The MC4R receptor plays an important role in regulating body weight and energy homeostasis. Knockout of the MC4R gene in HEK293T cells helps researchers understand the molecular mechanisms behind obesity and metabolic disorders.
Drug Screening and Development: HEK293T cells lacking MC4R can be used in high-throughput screening assays to identify potential agonists or antagonists against this receptor. Such compounds may lead to new treatments for obesity and related metabolic diseases.
Signal Transduction Studies: These cells provide a controlled environment to study downstream signaling pathways activated by MC4R. This is essential for elucidating the role of the receptor in various physiological processes and identifying nodes that may be targeted by therapeutic agents.
Genetic Studies: By comparing the behavior of MC4R knockout cells with wild-type cells, researchers can study the functions and interactions of genes. This comparative approach can reveal insights into gene networks and molecular players involved in energy balance.
Pharmacological Studies: MC4R knockout HEK293T cells are essential for studying the pharmacodynamics and pharmacokinetics of drugs targeting this receptor. These studies help understand drug-receptor interactions, drug efficacy and potential side effects, and guide the dosing and administration strategies of new therapies.
For research use only. Not intended for any clinical use.
