Human GALT Knockout Cell Line-HEK293T
Cat.No. : CSC-RT2741
Host Cell: HEK293T Target Gene: GALT
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2741 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human GALT using CRISPR/Cas9. |
| Target Gene | GALT |
| Host Cell | HEK293T |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid Nitrogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The GALT gene, also known as galactose-1-phosphate uridyltransferase, is essential for human metabolism. Galactose-1-phosphate uridyltransferase catalyzes a step in the metabolic pathway that breaks down galactose into glucose and other molecules that the body can use. Specifically, the enzyme converts galactose-1-phosphate into glucose-1-phosphate. The resulting glucose is the main energy source for cells. In addition, the process produces UDP-galactose, an essential molecule for the production of galactose-containing proteins and fats. These modified proteins and fats play an integral role in cell communication, structural integrity, molecular transport, and energy production.
Alterations or mutations in the GALT gene are associated with a metabolic disorder known as classic galactosemia. To date, more than 300 GALT gene mutations have been identified in individuals with classic galactosemia. The disorder appears shortly after birth and presents life-threatening symptoms due to the body's inability to process galactose effectively. This mutation often severely reduces or eliminates the activity of the galactose-1-phosphate uridyltransferase, leading to a toxic buildup of galactose-1-phosphate and related compounds in the body that damages tissues and organs.
Applications of Human GALT Knockout Cell Line-HEK293T
Galactose Metabolism Research: The main application of Human GALT Knockout Cell Line-HEK293T is to study galactose metabolism. The GALT gene encodes an enzyme that is essential for galactose metabolism. Disruption of this gene can help researchers understand metabolic pathways and their impacts.
Galactosemia Disease Modeling: Human GALT Knockout Cell Line is an important tool to model galactosemia, a genetic disease caused by deficiency of the GALT enzyme. By using this cell line, researchers can study the cellular and molecular mechanisms behind the disease and screen potential therapeutic agents.
Drug Development and Screening: Researchers can use Human GALT Knockout Cell Line-HEK293T for high-throughput screening of drugs. This cell line can be used to test the efficacy of compounds in compensating for the loss of GALT activity or alleviating the metabolic imbalance caused by its knockout.
Biochemical Pathway Analysis: These cell lines help to study the effects of GALT gene knockout on biochemical pathways in detail. Researchers can explore how GALT loss affects various cellular processes and identify potential biomarkers of metabolic dysfunction.
For research use only. Not intended for any clinical use.
