Human FAS Knockout Cell Line-PANC-1
Cat.No. : CSC-RT2709
Host Cell: PANC-1 Target Gene: FAS
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2709 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human FAS using CRISPR/Cas9. |
| Target Gene | FAS |
| Host Cell | PANC-1 |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid nirtogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The FAS gene, also known as Fas cell surface death receptor, CD95 or TNFRSF6, plays a crucial role in regulating apoptosis. It is a member of the tumor necrosis factor receptor (TNFR) family and is encoded by the FAS gene located on chromosome 10 in humans. The main function of the FAS receptor is to mediate apoptosis when it interacts with its ligand, FasL (Fas ligand). The binding of FasL to the FAS receptor triggers a complex series of intracellular signaling events that activate caspases, which are proteases that break down cells by degrading their proteins and other cellular components. This process is essential for the elimination of damaged or potentially harmful cells, such as those infected by viruses or transformed into cancer cells.
The role of the FAS is particularly important in the immune system, where it helps maintain immune tolerance and prevents the development of autoimmune diseases. For example, FAS-mediated apoptosis is essential for the deletion of autoreactive T cells, which would otherwise attack the body's own tissues. Defects or mutations in the FAS gene can lead to autoimmune lymphoproliferative syndrome (ALPS), which is characterized by the accumulation of lymphocytes and the development of autoimmune symptoms. In addition to its role in the immune system, the FAS pathway has been implicated in cancer. Some cancer cells evade apoptosis by downregulating FAS expression or function, allowing them to survive and proliferate unchecked. Conversely, therapies that can restore or enhance FAS-mediated apoptosis are being explored as potential treatments for cancer.
Human FAS Knockout Cell Line-PANC-1 is a genetically engineered cell line derived from the pancreatic cancer cell line PANC-1. This cell line has been modified using CRISPR-Cas9 technology to knockout the FAS gene, which encodes a protein involved in apoptosis signaling. Here are some of the applications of this cell line:
1. Cancer Research:
The FAS gene plays a crucial role in the regulation of apoptosis, and its dysfunction is often associated with cancer progression and resistance to therapy. By studying the Human FAS Knockout Cell Line-PANC-1, researchers can gain insight into the mechanisms by which pancreatic cancer cells evade apoptosis. This may lead to the identification of new therapeutic targets and strategies to sensitize cancer cells to therapy.
2. Drug Screening:
The Human FAS Knockout Cell Line-PANC-1 can be used in high-throughput drug screening assays to identify compounds that restore apoptotic pathways or specifically target cancer cells with defects in FAS signaling.
3. Signal Transduction Studies:
Scientists can use this knockout cell line to study the downstream effects of FAS gene loss on various signal transduction pathways. Understanding these pathways is critical for developing drugs that can modulate apoptotic signaling in cancer treatment.
4. Gene Function Analysis:
The human FAS knockout cell line - PANC-1 can be used as a model to study the effects of FAS gene loss of function in a controlled environment. Researchers can compare these knockout cells with wild-type cells to understand the specific role of FAS in cellular processes such as cell cycle regulation, proliferation, and apoptosis.
5. Study of drug resistance mechanisms:
Pancreatic cancer is known for its aggressiveness and resistance to traditional therapies. By utilizing FAS knockout cell lines, researchers can study the mechanisms behind this resistance, particularly focusing on the role of apoptosis evasion. This can help develop strategies to overcome treatment resistance.
For research use only. Not intended for any clinical use.
