Human EPX Knockout Cell Line-MOLM13
Cat.No. : CSC-RT2792
Host Cell: MOLM-13 Target Gene: EPX
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
Inquire for Price
Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2792 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human EPX using CRISPR/Cas9. |
| Target Gene | EPX |
| Host Cell | MOLM-13 |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid Nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Inquiry
Background
Applications
The EPX gene is located on human chromosome 17 and encodes eosinophil peroxidase (EPO), which is primarily expressed in eosinophils, a type of immune cell. Eosinophil peroxidase is a heme-containing enzyme that belongs to the class of oxidoreductases. Structurally, human eosinophil peroxidase consists of heavy and light chains formed by proteolytic processing of a precursor polypeptide. The enzyme contains an active site in which the heme cofactor is covalently attached. It has significant similarities to other peroxidases such as myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO). Its primary function is to catalyze the formation of hypohalous acid from hydrogen peroxide (H2O2) and halide ions, which is essential for the antimicrobial activity of the immune system.
Mutations in the EPX gene can lead to eosinophil peroxidase deficiency. Notably, at least three mutations have been identified that cause this deficiency. Despite the important role that eosinophil peroxidase plays in immune function, individuals with eosinophil peroxidase deficiency do not usually display any obvious health problems. These gene mutations may result in the complete absence of eosinophil peroxidase production or the production of a nonfunctional enzyme. As a result, the eosinophils of affected individuals may be small and structurally abnormal, but their overall function remains largely intact due to the presence of other functional proteins within the cell.
Applications of Human EPX Knockout Cell Line-MOLM13
1. Cancer Research: The Human EPX Knockout Cell Line-MOLM13 is a crucial tool in cancer research, particularly for studying acute myeloid leukemia (AML). EPX (Eosinophil Peroxidase) knockout models help researchers understand the role of EPX in the proliferation and survival of cancerous cells.
2. Drug Screening: By using the EPX Knockout Cell Line-MOLM13, researchers can test the efficacy and toxicity of new drugs, particularly those targeting pathways involving EPX. The differences in drug response between the knockout and wild-type cells can provide insights into the drug’s mechanism of action.
3. Gene Function Studies: EPX Knockout Cell Line-MOLM13 allows scientists to investigate the baseline functions of the EPX gene. By eliminating the gene, researchers can observe phenotypic changes, helping to elucidate the biological processes and pathways in which EPX is involved.
4. Immunology Research: This cell line is useful for studying the immune response, as EPX is involved in the immune system’s response to pathogens. Researchers can explore how the absence of EPX affects immune cell behavior, cytokine production, and overall immune response, potentially leading to new insights into immune disorders and inflammatory diseases.
For research use only. Not intended for any clinical use.
