Human DNMT3B Knockout Cell Line-HEK293T
Cat.No. : CSC-RT2710
Host Cell: HEK293T Target Gene: DNMT3B
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
Inquire for Price
Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2710 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human DNMT3B using CRISPR/Cas9. |
| Target Gene | DNMT3B |
| Host Cell | HEK293T |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid nirtogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Inquiry
Background
Applications
DNMT3B (DNA (cytosine-5)-methyltransferase 3 beta) encodes an enzyme that plays an important role in epigenetic regulation of the human genome. The enzyme is particularly important in the process of de novo methylation, which involves the addition of methyl groups to DNA cytosine residues. DNA methylation is a key epigenetic modification that contributes to a variety of cellular processes, including embryonic development, genomic imprinting, and X-chromosome inactivation. Modifications performed by DNMT3B can affect gene expression patterns by altering chromatin structure, making genes easier or harder to transcribe.
Mutations in the DNMT3B gene are associated with a rare genetic disorder called immunodeficiency-centromere instability-facial anomalies (ICF) syndrome. ICF syndrome is characterized by defects in lymphocyte maturation, centromere instability, and distinct facial anomalies. Recent studies have also implicated DNMT3B in various forms of cancer. Abnormal methylation patterns due to dysregulated DNMT3B function can lead to silencing of tumor suppressor genes and activation of oncogenes, which can lead to tumor initiation and progression. Studies have shown that overexpression of DNMT3B is frequently observed in several types of cancer, including lung, colon, and breast cancer.
Human DNMT3B knockout cell lines derived from HEK293T cells are powerful tools for epigenetic research, especially in studies investigating the role of DNA methylation. DNMT3B is one of the major DNA methyltransferases involved in establishing genomic DNA methylation patterns during development. Here are some of the key applications of DNMT3B knockout cell lines:
Cancer Research: Knockout cell lines help researchers understand the role of DNMT3B in tumorigenesis and cancer progression by revealing the consequences of its absence, thereby aiding in the development of targeted cancer therapies.
Genetic and Epigenetic Research: HEK293T cells with DNMT3B knockout facilitate the study of epigenetic modifications, such as DNA methylation, which play a crucial role in gene regulation, development, and disease. This provides further insight into gene expression patterns and the mechanisms underlying various genetic diseases.
Drug Screening and Development: These knockout cell lines provide a powerful platform for high-throughput drug screening. By evaluating the response of cells to different compounds in the absence of DNMT3B, researchers can identify potential drug candidates and evaluate their mechanisms of action, thereby aiding in the development of new therapeutic approaches.
Stem Cell Research: DNMT3B is essential for maintaining the pluripotency and differentiation potential of stem cells. Knockout lines are important models for studying how loss of DNMT3B affects stem cell maintenance and differentiation, providing crucial insights into regenerative medicine and developmental biology.
For research use only. Not intended for any clinical use.
