Human DNMT1/DNMT3B Double Knockout Cell Line-HEK293T
Cat.No. : CSC-RT2711
Host Cell: HEK293T Target Gene: DNMT1/DNMT3B
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2711 |
| Cell Line Information | This cell is a stable cell line with a homozygous double knockout of human DNMT1 and DNMT3B using CRISPR/Cas9. |
| Target Gene | DNMT1/DNMT3B |
| Host Cell | HEK293T |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid nirtogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
DNMT1 (DNA methyltransferase 1) is a protein-coding gene in humans that is responsible for transferring methyl groups to cytosine nucleotides in DNA. It plays a key role in maintaining methylation patterns after DNA replication, showing a preference for hemimethylated DNA. This methylation process is essential for epigenetic regulation and affects gene expression without changing the DNA sequence itself. This protein is also involved in a variety of molecular functions such as DNA binding and zinc ion binding. It plays a role in many biological processes, including DNA template transcription and cellular response to stimuli. Variations in the DNMT1 gene have been associated with diseases such as cerebellar ataxia, deafness, narcolepsy, and hereditary sensory neuropathy type IE.
DNMT3B (DNA methyltransferase 3 beta) is another protein-coding gene in humans that is essential for the CpG methylation process. Unlike DNMT1, DNMT3B is thought to play a role primarily in de novo methylation. The expression of DNMT3B is developmentally regulated and is primarily located in the nucleus. DNMT3B also plays an important role in other processes, such as transcriptional regulation and cellular responses to environmental factors. Defects or mutations in DNMT3B have been linked to a variety of diseases, including immunodeficiency-centromere instability-facial anomalies (ICF) syndrome, congenital heart defects, and various cancers.
The Human DNMT1/DNMT3B Double Knockout Cell Line - HEK293T is a powerful tool for a variety of research applications in the fields of epigenetics, cancer biology, and gene regulation. Here are some of the key applications:
Epigenetic Research: By eliminating DNMT1 and DNMT3B, researchers can effectively study the effects of disrupted methylation patterns on gene expression, revealing epigenetic regulation of various biological processes.
Cancer Research: Given the role of DNA methylation in cancer, this knockout cell line is valuable for studying the mechanisms by which aberrant methylation leads to carcinogenesis. It helps identify potential biomarkers and therapeutic targets that are unique to epigenetic modifications in cancer cells.
Gene Expression Analysis: Researchers can use high-throughput sequencing and other gene expression analyses to understand the broad effects of DNMT knockout on cellular function, providing insights into gene regulatory networks.
Drug Screening and Development: This cell line is an important model for screening drugs that target DNA methylation pathways. It enables pharmaceutical researchers to identify and evaluate compounds that can modulate methylation, providing potential treatments for diseases associated with epigenetic dysregulation.
Development of Epigenetic Therapies: Using this cell line, researchers can test the efficacy and specificity of new epigenetic therapies. This is particularly important for developing treatments for cancer and other diseases where DNA methylation patterns are disrupted.
For research use only. Not intended for any clinical use.
