Human CXCL11 Knockout Cell Line-A549
Cat.No. : CSC-RT2759
Host Cell: A549 Target Gene: CXCL11
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2759 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human CXCL11 using CRISPR/Cas9. |
| Target Gene | CXCL11 |
| Host Cell | A549 |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid Nitrogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The CXCL11 gene, also known as C-X-C motif chemokine ligand 11, encodes a protein that plays a key role in the immune system through chemokine activity. The gene is located on human chromosome 4 (4q21.1), contains four exons, and belongs to the CXC chemokine family. The CXCL11 protein acts primarily as a chemoattractant for activated T cells. It does this by binding to the chemokine receptor CXCR3 with high affinity, exceeding that of other ligands such as CXCL9 and CXCL10. The role of CXCL11 is not limited to immune cell chemotaxis, it is also involved in various biological processes, including leukocyte chemotaxis, T cell chemotaxis, chemokine-mediated signaling pathways, and response to lipopolysaccharide.
In addition, CXCL11 has clinical significance as a biomarker for heart failure and left ventricular dysfunction, along with CXCL9 and CXCL10. These chemokines have been implicated in the pathophysiological mechanisms of cardiac remodeling, making them key markers of cardiovascular disease. In addition, the role of this gene has been investigated in various cancers, highlighting its function in promoting or inhibiting tumor growth and metastasis through chemokine receptor interactions.
Applications of Human CXCL11 Knockout Cell Line-A549
1. Cancer Research: A549 cells are a model of lung adenocarcinoma, and knockout of CXCL11 helps researchers understand the role of chemokines in tumor progression, metastasis, and regulation of the tumor microenvironment. Knockout studies can elucidate pathways of tumor growth and aid in the development of targeted therapies.
2. Immunotherapy: By understanding how loss of CXCL11 affects the attraction and infiltration of immune cells in tumors, scientists can develop strategies to enhance the targeting of immune cells to cancer cells. This insight is critical to improving the efficacy of immune checkpoint inhibitors.
3. Drug Screening and Development: By testing a variety of compounds, researchers can identify new drugs that may modulate other pathways or proteins to counteract loss of CXCL11, thereby providing new treatments for diseases where this chemokine plays a key role.
4. Modeling Inflammatory Diseases: CXCL11 is involved in inflammatory responses. Researchers use knockout cell lines to study the underlying mechanisms of inflammatory diseases such as chronic obstructive pulmonary disease (COPD), asthma, and other respiratory diseases. This cell line helps understand disease pathology and identify potential anti-inflammatory drug targets.
5. Gene Regulation and Signaling Pathways: Researchers use this cell line to study gene regulation and signaling pathways affected by CXCL11 loss. By comparing gene expression profiles and signaling activity between knockout and wild-type cells, scientists can identify key regulatory networks and potential intervention points.
For research use only. Not intended for any clinical use.
