Human CXADR Knockout Cell Line-A549
Cat.No. : CSC-RT2762
Host Cell: A549 Target Gene: CXADR
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2762 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human CXADR using CRISPR/Cas9. |
| Target Gene | CXADR |
| Host Cell | A549 |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid Nitrogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
Coxsackievirus and adenovirus receptor (CXADR), also known as CAR, is a key protein encoded by the human CXADR gene. It functions primarily as a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. CAR is widely expressed in a variety of tissues, including the heart, brain, and various epithelial and endothelial cells. Functionally, CAR plays an integral role in cell-cell adhesion by interacting with extracellular matrix glycoproteins such as fibronectin, agrin, and laminin-1.
In cardiac tissue, CAR is essential for the proper electrical and mechanical coupling of cardiomyocytes at the intercalated disc. Studies have shown that knockout of CAR causes embryonic lethality in mice with severe cardiac abnormalities. In the central nervous system, CAR is involved in neural development and neurite outgrowth. Its expression is essential for the proper formation of lymphatic endothelial cell junctions. Since CAR is a receptor for coxsackie B virus and adenovirus, infection with these viruses can have severe consequences, especially in cardiac tissue, where they can lead to diseases such as myocarditis and dilated cardiomyopathy.
Applications of the Human CXADR Knockout Cell Line-A549:
Virology and Infectious Disease Research: By knocking out the CXADR gene, researchers can investigate how various viruses that utilize CXADR as a receptor, such as adenoviruses and Coxsackieviruses, interact with and enter host cells. This facilitates the development of antiviral strategies and therapeutic interventions.
Cancer Research: CXADR has been implicated in tumor suppression and cell adhesion. Utilizing the CXADR Knockout Cell Line-A549 enables scientists to explore the gene’s role in cancer progression, metastasis, and cell signaling pathways.
Drug Discovery and Development: The cell line serves as an invaluable tool in high-throughput screening for potential drug candidates. By examining the effect of various compounds on the CXADR-deficient A549 cells, researchers can identify molecules that specifically alter cellular pathways dependent on CXADR signaling, thereby accelerating the drug discovery process.
Gene Function and Mechanistic Studies: Researchers can study changes in cell adhesion, migration, and proliferation in the absence of CXADR, gaining deeper insight into the mechanistic roles of this gene in normal and pathological conditions.
Immunological Research: CXADR is involved in the immune response, particularly in modulating the activity of immune cells. The knockout cell line enables the study of immune evasion mechanisms employed by pathogens and cancer cells. This research can contribute to the development of immunotherapies and vaccines.
For research use only. Not intended for any clinical use.
