Human CRBN Knockout Cell Line-HEK293
Cat.No. : CSC-RT2706
Host Cell: HEK293 Target Gene: CRBN
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2706 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human CRBN using CRISPR/Cas9. |
| Target Gene | CRBN |
| Host Cell | HEK293 |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid nirtogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The CRBN (cereblon) gene is an essential component of cell biology with important implications for both health and disease. The gene is located on chromosome 3 at position 3p26.2. The cereblon protein encoded by the CRBN gene is found in the cytoplasm of cells and is part of the ubiquitin-proteasome system, a key pathway responsible for the degradation and recycling of proteins. One of the main functions of the CRBN protein is its role as a substrate receptor in an E3 ubiquitin ligase complex known as the CRL4 complex. This complex selectively tags certain proteins for proteasomal degradation through a process known as ubiquitination. Regulation of protein breakdown is essential for many cellular processes, including cell cycle progression, DNA repair, and signal transduction. Dysfunction of this pathway can lead to a variety of diseases, including cancer and neurodegenerative disorders.
The CRBN protein is also the target of the drug thalidomide, which gained notoriety in the 1950s and 1960s for its association with birth defects. Thalidomide and its derivatives lenalidomide and pomalidomide are immunomodulatory drugs used to treat multiple myeloma and other diseases. These drugs bind to the CRBN protein, altering its function and thereby affecting the substrate specificity of the CRL4 complex. This binding alters the pattern of protein degradation, which contributes to their therapeutic effects but also to their teratogenicity.
Human CRBN knockout cell line - HEK293 offers a wide range of applications in biomedical research and drug development:
Target identification and validation: This cell line helps identify and validate drug targets, especially for CRBN-related pathways. By knocking out the CRBN gene, researchers can study its specific role in various cellular processes, helping to elucidate drug mechanisms.
Functional genomics: CRBN knockout HEK293 cells are used for gene function studies, allowing researchers to study the downstream effects of CRBN loss on gene expression and phenotypic alterations. This is critical to understanding the broader biological significance of the gene.
Drug screening and development: The CRBN knockout cell line is an important tool for high-throughput screening of potential therapeutic compounds that interact with the CRBN pathway. It is particularly useful in the early stages of drug development to assess efficacy and specificity.
Mechanistic studies: Researchers use these cells to explore the molecular mechanisms of CRBN's role in cellular processes such as protein homeostasis, apoptosis, and signal transduction. These insights can provide a deeper understanding of disease pathogenesis and therapeutic intervention points.
Disease modeling: This cell line can be used as a model to study diseases associated with CRBN mutations or dysregulation, such as certain cancers and neurodevelopmental disorders. It can help elucidate disease mechanisms and test potential therapeutic strategies.
For research use only. Not intended for any clinical use.
