Human CNR2 Knockout Cell Line-HEK293T
Cat.No. : CSC-RT2712
Host Cell: HEK293T Target Gene: CNR2
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2712 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human CNR2 using CRISPR/Cas9. |
| Target Gene | CNR2 |
| Host Cell | HEK293T |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid nirtogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The CNR2 gene encodes cannabinoid receptor 2 (CB2), a member of the G protein-coupled receptor family. The primary ligands for the CB2 receptor are endocannabinoids such as 2-arachidonoylglycerol (2-AG). Activation of CB2 results in various intracellular effects, including inhibition of adenylate cyclase activity via the Gi/Goα subunit, which reduces intracellular cyclic adenosine monophosphate (cAMP) levels. This signaling pathway regulates a range of immune responses, including cytokine release, inflammation, and cell migration. In the immune system, CB2 receptors are expressed on cells such as B cells, T cells, monocytes, and macrophages. They help mediate immune responses and are involved in immune cell trafficking and retention, particularly in the spleen and lymph nodes.
In addition, CB2 receptors are known to play a role in regulating inflammation and analgesia, making them potential targets for the treatment of diseases such as chronic pain, inflammatory bowel disease, and neurodegenerative diseases. Recent studies have also highlighted a role for CB2 receptors in the central nervous system, where they are also evident on microglia and neurons, although they are expressed at a lower density than CB1 receptors. Activation of CB2 in the brain can influence neuroinflammation and neuroprotection, suggesting its relevance in diseases such as Alzheimer's disease and multiple sclerosis. The therapeutic potential of targeting CB2 receptors extends to a variety of clinical applications. For example, CB2 agonists have shown promise in reducing beta-amyloid plaque formation in Alzheimer's disease and modulating inflammation in cardiovascular disease.
The CNR2 gene encodes cannabinoid receptor type 2 (CB2), a component of the endocannabinoid system that is primarily expressed in immune cells but is also found in other tissues such as peripheral nerves. Knockout of the CNR2 gene in HEK293T cells provides a unique platform for studying the numerous physiological and pathological roles of the CB2 receptor. Below are a few key applications of this cell line.
1. Drug Target Validation:
By comparing drug responses in knockout and wild-type cells, researchers can determine the specificity and efficacy of new drug compounds. This is particularly important for drug development for diseases such as chronic pain, inflammation, and neurodegenerative diseases, where CB2 plays a crucial role.
2. Study Receptor Signaling Pathways:
Researchers can study how the loss of CNR2 affects various cellular processes, including G protein signaling, MAPK/ERK pathways, and calcium flux. These insights can provide a deeper understanding of how CB2 receptors affect cellular physiology and pathology.
3. Immune Response Analysis:
CB2 receptors are widely involved in immune regulation. Using CNR2 knockout HEK293T cells, scientists can dissect the molecular mechanisms by which CB2 receptors regulate immune cell function. This could provide new insights into therapeutic strategies for autoimmune diseases, infections, and cancer immunotherapy.
4. Cancer Research:
Emerging evidence suggests that CB2 receptors are involved in tumorigenesis and cancer progression. CNR2 knockout cell lines can be used to study the role of these receptors in cancer cell proliferation, apoptosis, and metastasis. This could lead to novel anticancer therapies targeting CB2 receptors.
For research use only. Not intended for any clinical use.
