Human ATM Knockout Cell Line-Hela
Cat.No. : CSC-RT2772
Host Cell: Hela Target Gene: ATM
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2772 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human ATM using CRISPR/Cas9. |
| Target Gene | ATM |
| Host Cell | Hela |
| Size Form | 1 vial (>10^6 cell/vial) |
| Shipping | Dry ice package |
| Storage | Liquid Nitrogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:4-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The ATM gene, also known as ATM serine/threonine kinase, plays a vital role in maintaining cellular health. It is located on chromosome 11 and provides instructions for the production of the ATM protein, which is primarily located in the cell nucleus. One of the main functions of the ATM protein is to help cells recognize and repair damaged or broken DNA strands. DNA damage can occur due to a variety of factors, such as toxic chemicals, radiation, or natural events when chromosomes exchange genetic material during cell division. The ATM protein coordinates the DNA repair process by activating enzymes that repair broken DNA strands, thereby maintaining the stability of the cell's genetic information. This is essential for preventing mutations and ensuring normal cellular functioning.
Mutations in the ATM gene can lead to a range of health problems, most notably Ataxia-Telangiectasia (A-T). This rare genetic disorder is characterized by difficulty coordinating movements (ataxia) that gradually develops starting in early childhood. Individuals with A-T have mutations in both copies of the ATM gene, resulting in the production of abnormally small and nonfunctional ATM proteins. As a result, cells are highly sensitive to radiation and fail to respond adequately to DNA damage, leading to the accumulation of mutations and potentially the development of cancerous tumors. In addition, carrying a single mutated copy of the ATM gene increases the risk of developing a variety of cancers, including breast, stomach, bladder, pancreatic, lung, and ovarian cancers.
Applications of Human ATM Knockout Cell Line - HeLa:
1. Cancer Research: The ATM (Ataxia Telangiectasia Mutated) gene plays a crucial role in the cellular response to DNA damage. Knockout HeLa cells lacking functional ATM are used to study the mechanisms of cancer development and progression, particularly regarding how cells respond to DNA damage and how carcinogenesis proceeds when such repair pathways are impaired.
2. Drug Testing and Development: This cell line aids in screening potential anti-cancer drugs. By understanding how ATM deficiency affects the sensitivity of cells to various compounds, researchers can identify and develop targeted therapies that are more effective against tumors carrying similar genetic defects.
3. Cell Cycle Regulation Studies: ATM is involved in cell cycle checkpoints, particularly after DNA damage. Studying ATM-deficient HeLa cells allows scientists to unravel the complexity of cell cycle regulation.
4. Signaling Pathway Analysis: ATM is a key player in various signaling pathways. Knockout cell lines allow exploration of the dynamics of these pathways, providing deeper insights into how cells transmit stress signals, repair damage or initiate apoptosis, thus broadening the understanding of cellular responses to various stimuli.
For research use only. Not intended for any clinical use.
