Human AHCYL1 Knockout Cell Line-HEK293

Cat.No. : CSC-RT2761

Host Cell: HEK293 Target Gene: AHCYL1

Size: 1x10^6 cells/vial, 1mL Validation: Sequencing

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RT2761
Cell Line Information	This cell is a stable cell line with a homozygous knockout of human AHCYL1 using CRISPR/Cas9.
Target Gene	AHCYL1
Host Cell	HEK293
Size Form	1 vial (>10^6 cell/vial)
Shipping	Dry ice package
Storage	Liquid Nitrogen
Species	Human

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Inquiry

Background

Applications

AHCYL1, also known as adenosylhomocysteinase-like 1, is a protein-coding gene in humans that encodes an enzyme with structure and function similar to adenosylhomocysteinase. The gene is located at position 13.3 of the short arm of chromosome 1 and is approximately 39 kilobases in length. The gene is expressed in various tissues including the brain, particularly in areas such as the globus pallidus, ventral tegmental area, and supravesicular nucleus. The AHCYL1 gene is involved in many important biological processes. It plays a crucial role in the S-adenosylmethionine cycle, which is essential for cellular metabolism and methylation reactions. This cycle is essential for regulating various physiological processes, including the methylation of DNA, RNA, proteins, and lipids.

AHCYL1 is known to interact with inositol 1,4,5-triphosphate (IP3) receptors, specifically ITPR1. This interaction is important for various cellular processes, including the calcium signaling pathway. Furthermore, AHCYL1 activity has been implicated in the regulation of mRNA polyadenylation and positive regulation of sodium ion transport, further highlighting its broad functional impact in cellular homeostasis.

The following are the main applications of the Human AHCYL1 Knockout Cell Line-HEK293:

Gene Function Analysis: The AHCYL1 knockout cell line allows researchers to explore the functional role of the AHCYL1 gene in cellular processes.

Disease Modeling: This cell line can be used to model diseases associated with mutations or dysregulation of the AHCYL1 gene. This helps understand the mechanisms underlying such diseases and aids in the development of targeted therapies and interventions.

Drug Screening: Researchers can use AHCYL1 knockout HEK293 cells to screen for drugs that target pathways affected by AHCYL1. This provides a platform for identifying potential therapeutic compounds and understanding their mechanisms of action.

Signal Transduction Studies: The AHCYL1 gene is involved in signaling pathways within cells. Knockout cell lines help reveal the role of AHCYL1 in these pathways and can lead to discoveries about cellular signaling mechanisms and their impact on health and disease.

Protein-Protein Interactions: The absence of AHCYL1 in these cells allows for the study of protein-protein interactions and helps identify interacting partners of AHCYL1.

For research use only. Not intended for any clinical use.