Custom sgRNA Expression Vector    

CRISPR/Cas9 Platform^CB's custom sgRNA vector is a high-quality selection that utilizes the CRISPR/Cas9 gene editing capabilities and the power of various expression systems to achieve a permanent 100% knockout in your cell line. The expression of sgRNA and Cas9 is stable and can be used for dividing or non-dividing cells or whole model organisms. Whether you need sgRNA expression for a knockout, knockin, point mutation, activation or inhibition, live-cell imaging or chromosomal immunoprecipitation, CRISPR/Cas9 Platform^CB can meet your requirements. Just tell us your need, we will design sgRNA, customize the CRISPR vector clone and (if needed) virus packaging for you!

Optional vectors

• Plasmid
• Adenovirus
• Lentivirus
• AVV

The vector contains an antibiotic gene and a fluorescent protein gene to ensure stability and to observe expression.

Our advantages

• We offer a variety of vector designs including sgRNA only, sgRNA-Cas9, dual sgRNA, inducible sgRNA vectors, etc.

➢ We offer Cas9 and dCas9 hybrids (e.g. dCas9-KRAB, dCas9-VPH, dCas9-VPR, etc.)
➢ We offer tet-inducible sgRNA construct designed for CRISPR KO, CRISPRa or CRISPRi.

• Our sgRNA expression vectors are ability to transfect a variety of cells, including difficult to transfect cells, such as stem cells, primary cells, suspension cells, etc.
• We offer two service modes

➢ Self-design - You provide the target sequence, selects the vector, and we finish the plasmid construction.
➢ CRISPR/Cas9 Platform^CB design - You provide the gene ID and we design the target sequence using a proprietary gRNA design tool.

CRISPR/Cas9^CB is committed to building effective systems and procedures to meet the needs and timelines of customers working in genome engineering. CRISPR/Cas9^CB provides customized sgRNA vector services, including expert research design, in vitro RNA synthesis and vector construction, enabling you to easily control target gene knockout, knockin, point mutation, DNA interaction analysis, live-cell imaging, etc. Our customized sgRNA vector service will help advance your genomics and protein function research projects and enhance your organization's ability to make informed decisions about important milestones.