Human TYK2 Knockout Cell Line-HEK293T
Cat.No. : CSC-RT0451
Host Cell: HEK293T Target Gene: TYK2
Size: >1x10^6 cells/vial Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT0451 |
| Cell Line Information | This cell line is a stable cell line with a homozygous knockout of human TYK2 using CRISPR/Cas9. |
| Target Gene | TYK2 |
| Gene ID | 7297 |
| Genotype | TYK2 (-/-) |
| Host Cell | HEK293T |
| Cell Type | Epithelial |
| Size | >1x10^6 cells/vial |
| Sequencing Result | Homozygous: 1 bp insertion in exon2 |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
TYK2 or tyrosine kinase 2 is an important enzyme that plays a key role in the signaling pathways of the immune system. TYK2 is essential for the proper functioning of various cytokine receptors, including interferons (IFNs), interleukins (ILs), and growth factor receptors. Specifically, it associates with receptors such as interferon-α/β receptor (IFNAR), interleukin-12 receptor (IL-12R), and interleukin-23 receptor (IL-23R). Through these associations, TYK2 regulates key biological processes such as cell proliferation, differentiation, apoptosis, and immune responses. This makes it a key mediator in both innate and adaptive immunity.
Given its important role in immunity and cell signaling, dysregulated TYK2 activity has been implicated in a variety of diseases. Overactive TYK2 signaling may contribute to autoimmune diseases such as rheumatoid arthritis, lupus, and psoriasis, which result in excessive or misguided immune responses. Conversely, insufficient TYK2 activity can lead to immune deficiency, making individuals more susceptible to infection. The importance of TYK2 also extends to therapeutic potential. Researchers are actively exploring TYK2 inhibitors as potential treatments for autoimmune diseases and certain cancers. By specifically targeting TYK2 and modulating its activity, these therapies aim to restore balance to the immune system without broadly suppressing immune function, thereby reducing potential side effects.
The 293T human embryonic kidney cell-based TYK2 (tyrosine kinase 2) knockout cell line provides a versatile tool for studying a variety of biological processes and disease mechanisms. Here are some important applications:
Drug screening: The TYK2 knockout cell line 293T can be an important tool for high-throughput drug screening. By eliminating the TYK2 kinase, researchers can identify compounds that selectively inhibit or reactivate pathways that depend on this enzyme. This is particularly valuable for developing targeted therapies for autoimmune diseases and certain cancers that are associated with TYK2.
Functional studies: This cell line can be used to perform in-depth functional studies of the TYK2 protein. By comparing TYK2 knockout 293T cells to wild-type, researchers can reveal the specific role of TYK2 in various signaling pathways, including its involvement in cytokine signaling and immune responses.
Pathway analysis: The TYK2 knockout 293T cell line provides a simplified model for studying downstream effectors and pathways activated by TYK2. This helps map the complete signaling cascade and understand the broader impact of TYK2 interactions and functions on cellular processes.
Genetic interaction studies: Using TYK2 knockout 293T cells, scientists can study genetic interactions and compensation of other kinases or proteins.
For research use only. Not intended for any clinical use.
