TFRC Knockout Cell Line-HeLa
Cat.No. : CSC-RT1891
Host Cell: Hela Target Gene: TFRC
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT1891 |
| Cell Line Information | This cell line is a stable cell line with a homozygous knockout of human TFRC using CRISPR/Cas9. |
| Target Gene | TFRC |
| Gene ID | 7037 |
| Genotype | TFRC (-/-) |
| Host Cell | Hela |
| Cell Type | Epithelial |
| Size | 1x10^6 cells/vial, 1mL |
| Sequencing Result | Homozygous: 1 bp insertion in exon 4 |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:4-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
Transferrin receptor (TfR), encoded by the TFRC gene, is a key protein involved in iron homeostasis. TfR is primarily found on the surface of proliferating cells and plays a key role in mediating cellular iron uptake. Molecularly, TfR is a transmembrane glycoprotein that binds with high affinity to transferrin, the major iron transporter in the blood. Each TfR binds two iron-loaded transferrin molecules and promotes their internalization via receptor-mediated endocytosis. After binding to transferrin, the receptor-transferrin complex is internalized into vesicles, which become acidic, resulting in the release of iron from transferrin. The iron is then transported into the cytoplasm, while apotransferrin and TfR are recycled back to the cell surface.
The expression of the TFRC gene is tightly regulated at all levels, including transcriptional and post-transcriptional mechanisms. The iron level in the cell regulates TFRC expression through iron-responsive elements (IREs) in mRNA that interact with iron regulatory proteins (IRPs). When iron is deficient, IRP binds to IRE and stabilizes TFRC mRNA, thereby promoting the production of TfR to promote more iron absorption. Clinically, changes in TFRC gene expression or TFRC function are associated with a variety of diseases. Overexpression of TFRC is often seen in rapidly proliferating cells, including cancer cells, making it a potential target for cancer treatment.
Creating a TFRC (transferrin receptor) knockout cell line using HeLa cells can be a powerful tool for a variety of research applications. Here are some key applications:
Iron metabolism research: TFRC plays a key role in cellular iron uptake. By knocking out TFRC in HeLa cells, researchers can study the effects of impaired iron uptake on cell physiology, metabolism, and viability.
Cancer research: HeLa cells are a widely used cancer cell line. TFRC knockout HeLa cells can help determine how disruption of iron homeostasis affects cancer progression and response to therapy.
Drug testing and development: TFRC is a potential target for drug development, especially for therapies targeting cancer and iron overload disorders. Using TFRC knockout HeLa cells in drug screening can help identify compounds that specifically target cells with impaired iron uptake mechanisms.
Cell signaling pathways: TFRC participates in multiple signaling pathways. Knockout cell lines can be used to study how loss of TFRC affects a variety of cell signaling processes, providing a clearer understanding of the receptor's role beyond iron acquisition.
Genetic studies: Creating a TFRC knockout in HeLa cells can help elucidate gene function and interactions. This approach could reveal genetic compensatory mechanisms that function when TFRC is lost, enhancing our understanding of cellular genetic networks.
For research use only. Not intended for any clinical use.
