SQSTM1 Knockout Cell Line-HCT116
Cat.No. : CSC-RT1874
Host Cell: HCT116 Target Gene: SQSTM1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT1874 |
| Cell Line Information | HCT116 -SQSTM1(-/-) is a stable cell line with a homozygous knockout of human SQSTM1 using CRISPR/Cas9. |
| Target Gene | SQSTM1 |
| Host Cell | HCT116 |
| Shipping | 1 vial of knockout cell line |
| Storage | Liquid nitrogen |
| Species | Human |
| Gene Symbol | SQSTM1 |
| Gene ID | 8878 |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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For research use only. Not intended for any clinical use.
