MTOR KO Cell Lysate-HEK293T

CLKO-0604

The cell lysate is obtained from the homozygous knockout cell line developed by CRISPR/Cas9.

Validate the specificity of antibodies.As a negative control for functional assay.

Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

HEK293T

Gene knockout cell lysates are the cell homogenate in RIPA buffer made with double-knockout cell lines. The Gene knockout cell lines are developed by CRISPR. The protein concentration was determined with BCA assay. A vial of lysate from the parental cell line was also included as an internal control.

KO cell lysate (100ug) and parental cell lysate (100ug)

HEK293T