MAPK14 Knockout Cell Line-293T
Cat.No. : CSC-RT0445
Host Cell: 293T Target Gene: MAPK14
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT0445 |
| Cell Line Information | 293T-MAPK14 (-/-) is a stable cell line with a homozygous knockout of human MAPK14 |
| Target Gene | MAPK14 |
| Host Cell | 293T |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
Mitogen-activated protein kinase 14, commonly referred to as MAPK14 or p38 α, is an important member of the MAP kinase family and plays a crucial role in cellular signaling. MAPK14/p38 α is encoded by the MAPK14 gene located on chromosome 6 in humans. Upon activation by the upstream kinases MKK3, MKK4, or MKK6, MAPK14 phosphorylates a range of substrates, including transcription factors, enzymes, and other protein kinases. This phosphorylation process converts external signals into appropriate cellular responses, ranging from differentiation, apoptosis, and autophagy to inflammation and cell cycle regulation. Due to the important role MAPK14 plays in these processes, it has become a focus of research in various diseases and conditions, especially inflammatory diseases, cancer, and neurodegenerative diseases.
One of the best-known functions of MAPK14 is its involvement in inflammatory responses. It regulates the production of proinflammatory cytokines such as IL-1, TNF-alpha, and IL-6, and also affects the activity of COX-2, an enzyme that is essential for prostaglandin synthesis. As a result, MAPK14 has become a target for anti-inflammatory drug development, and researchers are exploring p38 MAPK inhibitors as potential therapeutics for arthritis, Crohn's disease, and other inflammatory diseases.
The MAPK14 knockout cell line, derived from the human 293T cells, serves as a valuable tool in various research fields. Below are some prominent applications:
Gene Function Studies: By specifically knocking out the MAPK14 gene, researchers can investigate its role in cellular processes such as stress response, inflammation, cell cycle regulation, and apoptosis.
Drug Screening and Development: The MAPK14 pathway, also known as p38 MAP kinase pathway, is a critical component in inflammatory responses and various diseases, including cancer. This knockout cell line can be used to screen for compounds that target the MAPK14 pathway, aiding in the discovery and development of new therapeutic drugs.
Signal Transduction Research: MAPK14 is involved in transmitting extracellular signals to the intracellular machinery. By using the knockout cell line, researchers can dissect the intricacies of signaling pathways, identify compensatory mechanisms, and study the cross-talk between different signaling networks.
Cancer Research: Given the role of MAPK14 in regulating cellular growth and stress response, this knockout model can assist in elucidating its function in oncogenesis and tumor progression, providing insights that could lead to targeted cancer therapies.
For research use only. Not intended for any clinical use.
