Human SIK2 Knockout Cell Line-HeLa
Cat.No. : CSC-RT0838
Host Cell: HeLa Target Gene: SIK2
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT0838 |
| Cell Line Information | A stable cell line with a homozygous knockout of human SIK2 using CRISPR/Cas9. |
| Target Gene | SIK2 |
| Host Cell | HeLa |
| Shipping | 10^6 cells/tube |
| Storage | Liquid nitrogen |
| Species | Human |
| Gene ID | 23235 |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:4-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
SIK2 or Salt-Inducible Kinase 2 is a serine/threonine protein kinase that belongs to the AMP-activated protein kinase (AMPK) family. This kinase plays a crucial role in various cellular processes, such as metabolism, signal transduction, and gene expression. One of the main functions of SIK2 is to regulate hepatic gluconeogenesis. It does this by phosphorylating and inactivating CRTC2 (CREB-regulated transcriptional coactivator 2), which results in decreased transcription of gluconeogenic genes. This inactivation reduces glucose production in the liver, which is particularly beneficial for controlling hyperglycemia in diabetic patients. In addition, SIK2 plays a role in lipid metabolism. It is involved in regulating lipogenic gene expression in adipose tissue, affecting lipid biosynthesis and storage.
Recent studies have shown that SIK2 may act as a tumor suppressor in certain types of cancer, such as ovarian cancer. The mechanism involves SIK2's ability to regulate the cell cycle and apoptosis, thereby inhibiting tumor growth and proliferation. However, the role of SIK2 in cancer is complex and may vary depending on the cancer type and cellular context. Studies on SIK2 have also revealed its role in neuronal function and neuroprotection. It has been suggested that SIK2 may have therapeutic potential in the treatment of neurodegenerative diseases due to its role in neuronal survival and plasticity.
1. Cancer Research: SIK2 (Salt Inducible Kinase 2) is associated with various cellular processes such as metabolism, apoptosis, and cell cycle regulation. Knocking out SIK2 in HeLa cells can help elucidate its role in tumor progression and metastasis.
2. Drug Discovery: SIK2 knockout cells can aid in screening small molecule inhibitors or other compounds targeting pathways influenced by SIK2 activity, potentially leading to novel treatments for diseases where SIK2 plays a critical role.
3. Metabolic Studies: SIK2 has been implicated in the regulation of metabolic pathways. The knockout cell line is instrumental in studying the metabolic changes that occur when SIK2 is absent. Such studies can contribute to our understanding of metabolic disorders and provide insights into the development of therapies targeting metabolic imbalances.
4. Signal Transduction Research: SIK2 is part of important signaling pathways, including the AMPK pathway, which is crucial for cellular energy homeostasis. SIK2 knockout HeLa cells allow researchers to dissect the specific contributions of SIK2 to these signaling networks.
5. Apoptosis and Cell Survival Studies: By employing the SIK2 knockout cell line, scientists can explore how SIK2 influences cell survival and apoptosis. This is particularly relevant in the context of diseases characterized by dysregulated cell death, such as cancer and neurodegenerative diseases.
For research use only. Not intended for any clinical use.
