Human RB1 Knockout Cell Line-Hela
Cat.No. : CSC-RT0541
Host Cell: Hela Target Gene: RB1
Size: >1x10^6 cells/vial, 1 mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT0541 |
| Cell Line Information | This cell line is a stable cell line with a homozygous knockout of human RB1 using CRISPR/Cas9. |
| Target Gene | RB1 |
| Gene ID | 5925 |
| Genotype | RB1 (-/-) |
| Host Cell | Hela |
| Cell Type | Epithelial |
| Size | >1x10^6 cells/vial, 1 mL |
| Sequencing Result | Common region for coding isoform 1/2/3/4 Homozygous: 1 bp deletion in exon |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:4-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
Retinoblastoma 1, commonly referred to as RB1, is a gene that plays a key role in cell cycle regulation and is closely associated with tumorigenesis. RB1 is located on chromosome 13 (13q14.2) and encodes the retinoblastoma protein (pRB), which primarily functions as a tumor suppressor. The primary role of the retinoblastoma protein is to inhibit progression of the cell cycle from the G1 (first gap) phase to the S (synthesis) phase, where DNA replication occurs. pRB does this by binding to and inactivating the E2F transcription factor, which is essential for activating genes required for entry into the S phase. When pRB is functioning, it restricts cell growth and division, acting as a critical checkpoint to maintain cell proliferation and prevent uncontrolled growth that can lead to tumorigenesis.
Mutations or deletions in the RB1 gene can lead to a loss of pRB function, which results in uncontrolled cell division and growth. This loss of function is a key driver in the development of retinoblastoma. Children with hereditary retinoblastoma inherit one defective copy of the RB1 gene and develop cancer if the second copy mutates. Non-hereditary retinoblastoma involves somatic mutations in both copies of the RB1 gene within a single retinal cell. In addition to its role in retinoblastoma, RB1 mutations have been implicated in other types of cancer, such as osteosarcoma, small cell lung cancer, and bladder cancer. This widespread involvement highlights the importance of pRB in cellular regulation and cancer suppression.
The human RB1 knockout cell line - HeLa is a valuable tool in biomedical research with a range of important applications. Here are some of the main uses:
Cancer Research: This cell line is essential for studying the role of the RB1 gene in cancer development and progression. Since RB1 is a known tumor suppressor gene, its knockout provides insights into the mechanisms of cell cycle control and tumorigenesis.
Drug Screening: It is a valuable model for high-throughput drug screening. Researchers can identify and test potential therapeutic compounds that target pathways involved in RB1-deficient cancer cells.
Gene Function Studies: By using RB1 knockout HeLa cells, scientists can study the biological functions of the RB1 gene, including its interactions with other proteins, regulation of the cell cycle, and effects on genome stability.
Pathway Analysis: This cell line helps dissect the cell signaling pathways affected by RB1 loss. Understanding these pathways can lead to the discovery of biomarkers for early cancer detection and personalized medicine.
Therapeutic Development: Researchers can develop and validate new treatment strategies, such as gene therapy or targeted therapy, specifically for cancers with RB1 mutations or defects.
For research use only. Not intended for any clinical use.
