Human NOTCH2 Knockout Cell Line-HeLa
Cat.No. : CSC-RT0573
Host Cell: HeLa Target Gene: NOTCH2
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT0573 |
| Cell Line Information | A stable cell line with a homozygous knockout of human NOTCH2 using CRISPR/Cas9. |
| Target Gene | NOTCH2 |
| Host Cell | HeLa |
| Shipping | 10^6 cells/tube |
| Storage | Liquid nitrogen |
| Species | Human |
| Gene ID | 4853 |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:4-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
NOTCH2 is one of four members of the NOTCH receptor family (which also includes NOTCH1, NOTCH3, and NOTCH4) and is an essential component of the highly conserved Notch signaling pathway. This pathway plays a critical role in various cellular processes, including cell fate determination, differentiation, proliferation, and apoptosis. The NOTCH2 gene is located on chromosome 1p12 and encodes a single-pass transmembrane protein that contains multiple functional domains, including EGF-like repeats, LIN-12/Notch repeats (LNRs), and an intracellular region involved in signal transduction.
Functionally, NOTCH2 signaling is essential for the development and homeostasis of multiple tissues, including the nervous, cardiovascular, and immune systems. It is particularly important in regulating kidney and liver development. Disruption or mutation of the NOTCH2 gene has been associated with various human pathologies, including Alagille syndrome and Hajdu-Cheney syndrome. NOTCH2 mutations have also been associated with malignancies, such as B-cell lymphomas and carcinomas, highlighting its role in tumorigenesis.
Cancer Research: The human NOTCH2 knockout cell line (HeLa) plays an important role in cancer research. By eliminating the NOTCH2 gene, researchers can study the role of this gene in cancer cell proliferation, survival, and metastasis.
Drug Screening: NOTCH2 knockout HeLa cells are used in high-throughput drug screening to evaluate new compounds that may inhibit cancer progression.
Pathway Analysis: These cells are essential for studying the NOTCH signaling pathway. Researchers can map the interactions and effects of NOTCH2 loss on cellular function, revealing how this pathway contributes to various physiological processes and diseases.
Genetic Studies: Using NOTCH2 knockout HeLa cells, genetic interactions and compensatory mechanisms in the absence of NOTCH2 can be explored.
Stem Cell Research: NOTCH2 plays a role in maintaining stemness in certain cell types. By using NOTCH2 knockout HeLa cells, researchers can study the specific contribution of this gene to stem cell renewal and differentiation, which is essential for developing regenerative medicine approaches.
Developmental Biology: Notch signaling is fundamental for cell differentiation during development. Therefore, NOTCH2 knockout models in HeLa cells can reveal developmental abnormalities and potential interventions.
For research use only. Not intended for any clinical use.
