Human MECP2 Knockout Cell Line-HeLa
Cat.No. : CSC-RT0748
Host Cell: HeLa Target Gene: MECP2
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT0748 |
| Cell Line Information | A stable cell line with a homozygous knockout of human MECP2 using CRISPR/Cas9. |
| Target Gene | MECP2 |
| Host Cell | HeLa |
| Shipping | 10^6 cells/tube |
| Storage | Liquid nitrogen |
| Species | Human |
| Gene ID | 4204 |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:4-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
MECP2, or methyl-CpG binding protein 2, is a key gene that plays an important role in human neural development and function. Encoded by the MECP2 gene located on the X chromosome, the protein is widely expressed but is particularly abundant in the brain. The main function of MECP2 is to regulate genetic activity by binding to methylated CpG sites in DNA. This binding is essential for controlling the expression of genes involved in neural development, synaptic function, and plasticity. MECP2 can activate or repress target genes depending on the situation, thereby ensuring normal neural function. It plays an important role in the development of neurons, affecting their growth, maturation, and the formation of synapses.
Mutations in the MECP2 gene are most commonly associated with Rett syndrome, a severe neurodevelopmental disorder that primarily affects females due to its X-linked inheritance pattern. Rett syndrome is characterized by normal early growth followed by a period of developmental regression, resulting in severe cognitive and motor impairments, loss of purposeful hand use, and other neurological abnormalities. Given the critical function of this protein in the brain, mutations or defects in MECP2 have far-reaching consequences, leading to a wide range of neurological and developmental problems.
1. Neurological Disease Research: Mutations in MECP2 are predominantly associated with Rett Syndrome, a severe neurodevelopmental disorder. Using MECP2 knockout HeLa cells allows researchers to dissect the molecular and cellular mechanisms underlying Rett Syndrome and other MeCP2-related disorders, facilitating the development of potential therapeutic strategies.
2. Epigenetic Studies: MeCP2 is a key player in the epigenetic regulation of gene expression through its ability to bind methylated DNA and recruit other regulatory proteins. MECP2 knockout HeLa cells provide a valuable system to study the effects of the loss of MeCP2 on global and gene-specific DNA methylation patterns, histone modification states, and the resultant transcriptomic changes.
3. Drug Screening and Development: The MECP2 knockout HeLa cell line is a powerful tool for high-throughput drug screening aimed at identifying compounds that can compensate for MECP2 loss or modulate its downstream effects.
4. Functional Genomics: MECP2 knockout HeLa cells can help in the identification and validation of MeCP2 target genes, pathways, and interaction networks. Such studies can provide insights into the broader biological roles of MeCP2 in cellular processes and pathways.
5. Cancer Research: Emerging studies suggest that MECP2 may play a role in certain cancers, influencing cell proliferation, apoptosis, and differentiation. The MECP2 knockout HeLa cell line can be used to examine how the absence of MeCP2 affects oncogenic processes and to identify dependencies that could be targeted in cancer therapies.
For research use only. Not intended for any clinical use.
