Human LRP6 Knockout Cell Line-HeLa
Cat.No. : CSC-RT0763
Host Cell: HeLa Target Gene: LRP6
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT0763 |
| Cell Line Information | A stable cell line with a homozygous knockout of human LRP6 using CRISPR/Cas9. |
| Target Gene | LRP6 |
| Host Cell | HeLa |
| Shipping | 10^6 cells/tube |
| Storage | Liquid nitrogen |
| Species | Human |
| Gene ID | 4040 |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:4-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
Low-density lipoprotein receptor-related protein 6 (LRP6) is a key protein involved in multiple cellular processes, including lipid metabolism, signal transduction, and cell development. Encoded by the LRP6 gene located on chromosome 12p13.2, the protein plays an important role in the canonical Wnt signaling pathway, which is essential for embryonic development, cell proliferation, and differentiation. In Wnt signaling, LRP6 forms a complex with the Frizzled receptor upon Wnt ligand binding. This interaction triggers the phosphorylation of LRP6, creating docking sites for other signaling molecules such as Disheveled (Dvl) and Axin. The subsequent stabilization of β-catenin and its translocation into the nucleus leads to the transcription of Wnt target genes, thereby affecting cell fate, proliferation, and migration. LRP6-mediated dysregulation of Wnt signaling has been implicated in various pathologies, including cancer, osteoporosis, and neurodegenerative diseases.
In addition to its role in Wnt signaling, LRP6 is also involved in lipid metabolism by regulating the clearance of plasma lipoproteins. Studies have shown that mutations in the LRP6 gene can lead to severe metabolic disorders such as familial hypercholesterolemia and coronary artery disease, highlighting its importance in maintaining metabolic homeostasis. Its importance in development and pathology makes it an important topic for biomedical research. Understanding the mechanistic basis of LRP6 function could pave the way for targeted therapeutic interventions in related diseases.
Because the LRP6 gene plays an important role in a variety of cellular and physiological processes, creating a human LRP6 knockout cell line in HeLa cells can be used for a variety of research applications. Here are some important applications:
Cancer Research: By observing the effects of LRP6 loss, researchers can gain insight into how LRP6 promotes tumor progression, metastasis, and cell proliferation, allowing for the development of targeted cancer therapies.
Wnt Signaling Pathway Analysis: LRP6 is an essential coreceptor in the Wnt signaling pathway. Knockout cell lines allow scientists to study the specific functions of LRP6 in this pathway and understand its role in cellular processes such as differentiation, migration, and growth.
Drug Testing and Development: Using LRP6 knockout HeLa cells in high-throughput screening assays can identify potential therapeutic compounds. These cells provide a platform to test the efficacy and safety of drugs that may modulate the Wnt signaling pathway or other pathways affected by LRP6 loss.
Genetic and Epigenetic Studies: This cell line can be used to explore genetic and epigenetic changes that occur due to LRP6 loss. Researchers can study gene expression profiles, chromatin modifications, and other alterations to gain deeper insights into gene regulation mechanisms.
Elucidation of Biological Mechanisms: By studying the downstream effects of LRP6 knockout, scientists can uncover the broader biological mechanisms and cellular responses involved. This helps elucidate how cells compensate for the loss of LRP6 function and may identify other key pathways and interactions.
For research use only. Not intended for any clinical use.
