Human IGF1R Knockout Cell Line-Hela
Cat.No. : CSC-RT0560
Host Cell: Hela Target Gene: IGF1R
Size: >1x10^6 cells/vial, 1mL Validation: Sequencing
Inquire for Price
Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT0560 |
| Cell Line Information | This cell line is a stable cell line with a homozygous knockout of human IGF1R using CRISPR/Cas9. |
| Target Gene | IGF1R |
| Gene ID | 3480 |
| Genotype | IGF1R (-/-) |
| Host Cell | Hela |
| Cell Type | Epithelial |
| Size | >1x10^6 cells/vial, 1mL |
| Sequencing Result | Homozygous: 100 bp deletion in exon |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:4-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Inquiry
Background
Applications
The insulin-like growth factor 1 receptor (IGF1R) is a transmembrane receptor that plays a key role in growth and development, particularly in cell proliferation, differentiation, and survival. IGF1R is widely expressed in various tissues and is activated by binding to its major ligand, insulin-like growth factor 1 (IGF-1). This binding triggers autophosphorylation of the receptor, which subsequently triggers phosphorylation of intracellular substrates such as insulin receptor substrates (IRS) and Shc proteins. These events activate downstream signaling pathways, particularly the PI3K/Akt and MAPK pathways, which are essential for mediating the biological effects of IGF1R activation.
Once activated, the PI3K/Akt pathway promotes cell survival and growth by inhibiting apoptotic processes and stimulating protein synthesis. On the other hand, the MAPK pathway affects cell division and differentiation. Due to its important role in these critical cellular functions, IGF1R has also been implicated in various pathological conditions, including cancer. Overexpression or overactivation of IGF1R has been observed in many tumor types, making it an important target for cancer therapy. Researchers are actively investigating IGF1R inhibitors and antibodies that can block its activity with the goal of developing effective cancer treatments.
1. Cancer Research: IGF1R is known to play a significant role in the proliferation, survival, and metastasis of cancer cells. By knocking out IGF1R, researchers can investigate the role and mechanisms of this receptor in various cancer types, leading to potential new therapeutic targets.
2. Drug Development and Testing: The IGF1R knockout HeLa cell line can be used to evaluate the efficacy of new anticancer drugs targeting the IGF signaling pathway. The absence of IGF1R in these cells helps to highlight the specificity and potential off-target effects of these drugs.
3. Signaling Pathway Analysis: HeLa cells with an IGF1R knockout can be utilized to dissect the IGF1 signaling pathway. By comparing these cells to wild-type HeLa cells, researchers can identify downstream effectors and alternative pathways that compensate for the loss of IGF1R.
4. Metabolic Studies: IGF1R is also implicated in metabolic regulation. Using IGF1R knockout HeLa cells, scientists can study how the absence of this receptor affects cellular metabolism, including glucose uptake, insulin sensitivity, and lipid metabolism.
5. Apoptosis and Cell Survival Studies: The IGF1R pathway is known to be involved in cell survival and apoptosis (programmed cell death). IGF1R knockout HeLa cells provide a model to study the intrinsic and extrinsic factors influencing these processes.
6. Immune Response and Inflammation: Some studies suggest that IGF1R is involved in modulating immune responses and inflammation. IGF1R knockout HeLa cells can help elucidate these roles by investigating how this receptor influences inflammatory signaling pathways and cytokine production, which is especially relevant in research focused on chronic inflammatory diseases and cancer.
For research use only. Not intended for any clinical use.
