Human FOXO4 Knockout Cell Line-Hela
Cat.No. : CSC-RT2398
Host Cell: Hela Target Gene: FOXO4
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2398 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human FOXO4 using CRISPR/Cas9. |
| Target Gene | FOXO4 |
| Host Cell | Hela |
| Storage | Liquid nirtogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:4-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
FOXO4 is a member of the forkhead box O (FOXO) family of transcription factors that plays a key role in regulating various cellular processes, including apoptosis, cell cycle control, DNA repair, and protection against oxidative stress. The FOXO family is characterized by the presence of a conserved "forkhead" DNA-binding domain, which enables these transcription factors to bind to specific DNA sequences and regulate the expression of target genes. One of the main roles of FOXO4 is to mediate the cellular response to oxidative stress. It does this by upregulating the expression of genes involved in the detoxification of reactive oxygen species (ROS) and the repair of oxidative damage. For example, FOXO4 induces the expression of manganese superoxide dismutase (MnSOD) and catalase, enzymes that are essential for neutralizing ROS.
FOXO4 also plays a key role in cellular lifespan and senescence. In senescent cells, FOXO4 interacts with p53, a key regulator of the cell cycle and apoptosis, to promote cell cycle arrest and prevent the proliferation of damaged cells. In addition, FOXO4 has been implicated in cancer biology. Depending on the context, it can function as a tumor suppressor or oncogene. In some cancers, FOXO4 inactivation leads to uncontrolled cell proliferation and survival, while in other cancers its activity may contribute to resistance to chemotherapy and radiation therapy. Understanding the dual role of FOXO4 in cancer is an active topic of research and has the potential to develop therapeutic interventions targeting the FOXO4 signaling pathway.
Here are some key applications of the human FOXO4 knockout cell line - HeLa:
Cancer research: FOXO4 is involved in various cellular processes, including cell cycle regulation, apoptosis, and oxidative stress responses. By studying the behavior of HeLa cells in the absence of this gene, researchers can gain insight into the mechanisms of tumorigenesis and cancer progression.
Drug screening: This knockout cell line can serve as a valuable tool for high-throughput drug screening. Because FOXO4 has been implicated in pathways that affect cell survival and proliferation, this cell line can be used to test the efficacy of novel anticancer drugs, thereby facilitating the development of targeted therapies.
Aging research: FOXO4 plays a role in the cellular aging process. The loss of FOXO4 in HeLa cells provides a model for studying the molecular pathways that control aging and senescence, potentially leading to the discovery of interventions that can delay aging or mitigate age-related diseases.
Gene interaction studies: The FOXO4 knockout HeLa cell line allows for the exploration of gene interaction networks. By analyzing the cell's response to the knockout, researchers can identify additional genes and signaling pathways that interact with FOXO4, revealing new targets for genetic or pharmacological intervention.
Oxidative stress research: FOXO4 is involved in the cellular response to oxidative stress. Using knockout cell lines, scientists can study the specific pathways and factors regulated by FOXO4 under conditions of oxidative stress, which can help to better understand diseases associated with oxidative damage, such as neurodegenerative and cardiovascular diseases.
For research use only. Not intended for any clinical use.
