Human FBXW7 Knockout Cell Line-DLD-1
Cat.No. : CSC-RT0018
Host Cell: DLD-1 Target Gene: FBXW7
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT0018 |
| Cell Line Information | DLD-1-FBXW7 (-/-) is a cell line with a homozygous knockout of human FBXW7 |
| Target Gene | FBXW7 |
| Host Cell | DLD-1 |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
FBXW7, also known as F-box/WD repeat protein 7, is an essential component of the SCF (Skp1-Cul1-F-box protein) ubiquitin ligase complex. This protein complex plays important roles in cellular processes by promoting ubiquitination and subsequent proteasomal degradation of specific substrate proteins. FBXW7 is characterized by the presence of an F-box domain, which is responsible for binding to Skp1, and multiple WD40 repeats that mediate substrate recognition. By targeting various substrates for degradation, FBXW7 regulates key cellular functions, including cell cycle progression, cell growth, and apoptosis. It functions as a tumor suppressor by controlling the stability of several oncogenic proteins, such as cyclin E, c-Myc, Notch1, and mTOR.
The importance of FBXW7 in cancer biology lies in the fact that this gene is frequently mutated in multiple types of cancer, including colorectal, breast, and liver cancers. Mutations often impair its substrate binding ability, leading to the accumulation of oncogenic proteins and uncontrolled cell proliferation. This suggests that dysfunctional FBXW7 can significantly promote tumorigenesis. Recent studies have also shown that FBXW7 plays a role in metabolic homeostasis, highlighting its multifaceted impact on human health. In addition, FBXW7 is emerging as a potential therapeutic target. Compounds that restore mutant FBXW7 function or enhance its activity could provide new avenues for cancer treatment.
Applications of Human FBXW7 Knockout Cell Line-DLD-1
Cancer Research: FBXW7 is a tumor suppressor often mutated in various cancers. DLD-1 cells are a colorectal carcinoma cell line, making this knockout model particularly relevant for studying colorectal cancer. Researchers can investigate how FBXW7 loss contributes to tumor development, progression, and metastasis.
Drug Screening: The knockout cell line can be used in high-throughput screening to identify small molecules or drugs that can selectively kill FBXW7-deficient cells, providing potential therapeutic avenues for FBXW7-mutant cancers.
Signal Transduction Studies: Understanding the role of FBXW7 in cell signaling pathways such as Notch, mTOR, and c-Myc.
Gene Function Studies: Comparing gene expression profiles between wild-type and FBXW7 knockout DLD-1 cells can reveal the downstream effects and functional outcomes of FBXW7 loss.
Biomarker Discovery: Using FBXW7 knockout DLD-1 cells can assist in identifying potential biomarkers for predicting the response to therapies, particularly in colorectal cancer patients harboring FBXW7 mutations.
For research use only. Not intended for any clinical use.
