Human BRCA2 Knockout Cell Line-DLD-1
Cat.No. : CSC-RT0015
Host Cell: DLD-1 Target Gene: BRCA2
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT0015 |
| Cell Line Information | DLD-1-BRCA2 (-/-) is a cell line with a homozygous knockout of human BRCA2 |
| Target Gene | BRCA2 |
| Host Cell | DLD-1 |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
BRCA2 (breast cancer susceptibility protein type 2) is an important gene in the human genome that plays an important role in maintaining genomic stability. BRCA2 is located on chromosome 13 and encodes a large protein consisting of approximately 3,418 amino acids that is essential for repairing DNA damage via homologous recombination. This gene is critical because mutations within BRCA2 are strongly associated with an increased risk of developing hereditary breast and ovarian cancer, as well as other malignancies.
The main function of BRCA2 is to promote the accurate repair of double-strand DNA breaks, which are one of the most lethal types of DNA damage. It does this by controlling the RAD51 protein, a key player in homologous recombination. By helping homologous DNA sequences to align and pair correctly, BRCA2 ensures that any double-strand breaks are accurately repaired, thereby preventing genomic instability that can lead to cancer. BRCA2 gene mutations can be inherited in an autosomal dominant manner, increasing an individual's risk of developing cancer. Women who carry a harmful BRCA2 mutation have a lifetime risk of developing breast cancer of up to 70%. Men with BRCA2 mutations are also at increased risk, particularly for prostate and pancreatic cancers.
The Human BRCA2 Knockout Cell Line-DLD-1 is a powerful tool in the study of cancer biology, genetic research, and drug development. Here are some key applications of this cell line:
Cancer Research: BRCA2 knockout DLD-1 cells are utilized to study the mechanisms of cancer development and progression, particularly in breast and ovarian cancers.
Drug Screening: These cells are instrumental in high-throughput screening of potential cancer therapeutics and assessing drug efficacy and resistance.
Genomic Studies: Researchers use these cells to investigate the role of BRCA2 in DNA repair, genome stability, and the cellular response to DNA damage.
Functional Analysis: The cell line serves as a model to explore BRCA2's functions and interactions with other proteins involved in cell cycle regulation and tumor suppression.
Gene Editing: These cells are a valuable tool for CRISPR/Cas9 and other gene editing technologies to assess the impact of BRCA2 mutations and to engineer new genetic models.
For research use only. Not intended for any clinical use.
