Human ATF4 Knockout Cell Line-HEK293T
Cat.No. : CSC-RT2337
Host Cell: HEK293T Target Gene: ATF4
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2337 |
| Cell Line Information | This cell is a stable cell line with a homozygous knockout of human ATF4 using CRISPR/Cas9. |
| Target Gene | ATF4 |
| Host Cell | HEK293T |
| Storage | Liquid nirtogen |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
The ATF4 gene, also known as activating transcription factor 4, is a key component of the cellular response to various stress conditions. ATF4 is located on human chromosome 22q13.1 and belongs to the ATF/CREB family of transcription factors, which play a key role in regulating gene expression in response to environmental and physiological stressors. ATF4 is known for its involvement in the integrated stress response (ISR), a cellular defense mechanism that regulates protein synthesis under stress conditions such as nutrient deprivation, oxidative stress, and endoplasmic reticulum (ER) stress. Under normal conditions, ATF4 expression is low. However, in response to stress, ATF4 levels increase significantly, initiating gene transcription that helps restore cellular homeostasis. Key processes regulated by ATF4 include amino acid metabolism, antioxidant response, and apoptosis.
In addition, ATF4 plays a vital role in developmental processes and cell differentiation. In particular, it plays an important role in osteoblast differentiation and bone formation. Disruption of ATF4 function has been associated with skeletal development disorders and other metabolic abnormalities. In the context of cancer, ATF4 exhibits a dual role. On the one hand, it can promote tumor cell survival under stress conditions, thereby promoting chemoresistance and cancer progression. On the other hand, overactivation of ATF4 can lead to cell death, providing a potential therapeutic window. This duality has made ATF4 a focus of cancer research, especially the development of targeted therapies that can modulate its activity to treat various malignancies.
Here are some key applications of the Human ATF4 Knockout Cell Line - HEK293T:
Gene Function Studies: By knocking out ATF4, researchers can study the role of this gene in cellular processes such as stress response, gene expression regulation, and metabolic pathways.
Cancer Research: ATF4 has been implicated in various cancers and can play a role in tumor growth and survival under stress conditions. Using the ATF4 knockout cell line can help determine its contribution to the carcinogenic process and may aid in the development of new therapeutic strategies.
Protein Interactions: This cell line can be used to study protein-protein interactions involving ATF4. The loss of ATF4 can elucidate its interaction networks and how these networks are perturbed in different physiological or pathological settings.
Drug Screening and Development: The ATF4 knockout cell line can be a valuable tool in pharmacological studies to screen for drugs that may specifically target pathways involving ATF4. This can inform the development of new drugs for diseases where ATF4 is a key factor.
Neurobiology: Because ATF4 is involved in the cellular response to oxidative and endoplasmic reticulum stress, this knockout cell line can be used to study neurodegenerative diseases and other diseases where these stress responses are critical.
Metabolic Studies: ATF4 is known to regulate genes involved in amino acid metabolism and cellular adaptation to nutrient deprivation. This cell line can aid in the exploration of metabolic disorders and biochemical pathways affected by ATF4.
For research use only. Not intended for any clinical use.
