Human AKT1 Knockout Cell Line-HCT116
Cat.No. : CSC-RT0061
Host Cell: HCT116 Target Gene: AKT1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
Inquire for Price
Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT0061 |
| Cell Line Information | HCT116 -AKT1 (-/-) is a cell line with a homozygous knockout of human AKT1 |
| Target Gene | AKT1 |
| Host Cell | HCT116 |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Inquiry
Background
Applications
AKT1, also known as protein kinase B (PKB), is a key enzyme in cell signaling pathways and plays a critical role in regulating various cellular processes. AKT1 belongs to the family of serine/threonine-specific protein kinases and is a key player in the PI3K/AKT/mTOR pathway, one of the most important and extensively studied signaling pathways involved in cell growth, proliferation, survival, and metabolism. When cells are stimulated by a range of growth factors, hormones, and other extracellular signals, the AKT1 protein is activated through phosphorylation. Upon activation, AKT1 translocates to different cellular compartments and phosphorylates various downstream substrates. This phosphorylation cascade can produce a variety of biological effects, including enhanced glucose uptake, increased protein synthesis, and inhibition of apoptosis, thereby promoting cell survival and growth.
The role of AKT1 in cell survival is particularly prominent in cancer. Abnormal activation of AKT1 is common in various types of cancer, resulting in uncontrolled cell proliferation and resistance to programmed cell death. This overactivation can result from mutation, amplification, or loss of negative regulators such as PTEN, a phosphatase that negatively regulates the PI3K/AKT pathway. As a result, AKT1 is considered an important oncogene, and the PI3K/AKT pathway has become a focus of cancer research and therapeutic intervention. Several inhibitors targeting different components of this pathway are currently in development or clinical use, aiming to prevent cancer progression by disrupting aberrant AKT1 signaling.
The AKT1 gene encodes a serine/threonine kinase that plays a key role in regulating a variety of cellular processes, including growth, survival, and metabolism. The human AKT1 knockout (KO) cell line-HCT116 provides a valuable tool for scientific research. Here are some of the key applications of this KO cell line:
Cancer Research: AKT1 is frequently implicated in cancer due to its role in cell survival, proliferation, and metabolism. Knocking out AKT1 in HCT116 cells can help researchers understand its exact role in cancer progression and therapeutic resistance.
Drug Discovery and Screening: AKT1 knockout cell lines can be used to screen for potential therapeutic compounds that target the AKT pathway or to identify off-target effects of drugs on cell signaling and function.
Cell Signaling Studies: Researchers can study how loss of AKT1 affects other pathways, such as PI3K, mTOR, and GSK3, to gain insight into broader cellular impacts.
Metabolism Studies: AKT1 is involved in glucose metabolism and cell growth. The use of knockout cell lines facilitates the study of metabolic changes, which is important for understanding diseases such as diabetes and metabolic syndrome.
Apoptosis and Cell Survival: Since AKT1 is known to transmit cell survival signals, this cell line is ideal for studying apoptosis.
Genetic Interaction Studies: The AKT1 knockout HCT116 cell line can be used to explore genetic interactions and epistasis by identifying compensatory pathways or synthetic lethality, thereby revealing new therapeutic targets or biomarkers.
For research use only. Not intended for any clinical use.
