GS/Bax/Bak1 Knockout Cell Line-CHO-K1
Cat.No. : CSC-RT0103
Host Cell: CHO-K1 Target Gene: GlulBaxBak1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT0103 |
| Cell Line Information | CHO-K1-GS/Bax/Bak cell line is a stable cell line with a homozygous knockout of GS, Bax and Bak1 |
| Target Gene | GlulBaxBak1 |
| Host Cell | CHO-K1 |
| Species | Hamster |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
Glutamine synthetase (GS):
Glutamine synthetase (GS) is an enzyme that catalyzes the conversion of glutamate and ammonia to glutamine, an essential amino acid. Although GS is primarily recognized for its role in nitrogen metabolism, emerging evidence suggests that GS also affects apoptotic pathways. Changes in glutamine levels regulated by GS affect the redox state and bioenergetics of the cell, indirectly regulating stress responses and apoptosis. Elevated GS activity is associated with cell survival and resistance to apoptosis under specific physiological and pathological conditions, including certain cancers.
Bcl-2-associated X protein (Bax):
Bax is a pro-apoptotic member of the Bcl-2 protein family that plays a key role in the mitochondrial pathway of apoptosis. Upon receipt of pro-apoptotic signals, Bax undergoes conformational changes and translocates to the outer mitochondrial membrane. Here, it oligomerizes to form pores that facilitate the release of cytochrome c and other apoptotic factors into the cytosol. This release triggers the activation of the apoptotic executioner caspases. The activity of Bax is tightly regulated by various other Bcl-2 family proteins, such as the anti-apoptotic Bcl-2 and Bcl-xL, which inhibit its pro-apoptotic function, thus maintaining the balance between cell survival and death.
BCL2-antagonist/killer 1 (Bak1):
Bak1 is another pro-apoptotic member of the Bcl-2 family that shares significant functional and structural similarities with Bax. It is also localized to the mitochondrial membrane and promotes cytochrome c release via pore formation upon activation. Unlike Bax, which is primarily in the cytoplasm until activated, Bak1 is primarily anchored in the mitochondrial membrane even in healthy cells, suggesting a poised state, ready to respond rapidly to apoptotic signals. This localization allows Bak1 to rapidly amplify apoptotic signals once initiated.
GS/Bax/Bak1 knockout cell lines derived from CHO-K1 cells are powerful tools in various fields of biomedical research and biotechnology. The simultaneous knockout of the GS (glutamine synthetase), Bax (Bcl-2 associated X protein), and Bak1 (Bcl-2 homologous antagonist killer 1) genes in these cells opens up a wide range of applications:
Protein production: The CHO-K1 cell line with GS, Bax, and Bak1 knockout is used for high-yield protein production in biopharmaceuticals. Due to its stability in culture and enhanced survival, this engineered cell line is optimized for industrial-scale production of therapeutic proteins, monoclonal antibodies, and other recombinant proteins.
Apoptosis research: This cell line is essential for apoptosis research, especially pathways involving Bax and Bak1. By knocking out these genes, researchers can study alternative apoptotic mechanisms and the role of these proteins in cell death, thus aiding the study of cancer and neurodegenerative diseases.
Drug Screening: It provides a reliable model for high-throughput screening of drugs and compounds that target apoptotic pathways or are designed to enhance cell viability.
Gene Function Studies: Researchers can use this knockout cell line to study gene functions and interactions. Specifically, the roles of GS, Bax, and Bak1 in cellular metabolism, stress response, and survival mechanisms can be explored without interference from these genes.
Metabolic Engineering: By understanding and manipulating metabolic pathways, the production of desired biomolecules can be increased, making the process more efficient and cost-effective for industrial applications.
Viral Production: This cell line is also used to produce viral vectors for gene therapy. The modified apoptotic pathway ensures a higher yield of viral particles, as the cells are less likely to die during the production phase, thereby increasing the efficiency of gene therapy vector manufacturing.
For research use only. Not intended for any clinical use.
