EEF1B2 Knockout Cell Line-293T
Cat.No. : CSC-RT0391
Host Cell: 293T Target Gene: EEF1B2
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT0391 |
| Cell Line Information | 293T-EEF1B2 (-/-) is a stable cell line with a homozygous knockout of human EEF1B2 |
| Target Gene | EEF1B2 |
| Host Cell | 293T |
| Species | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
EEF1B2, or eukaryotic translation elongation factor 1 Beta 2, is a key gene that plays an important role during protein synthesis. This gene belongs to the elongation factor family and is fundamentally involved in the elongation phase of protein translation, one of the most important processes in cell biology, in which proteins are synthesized from the amino acid sequence dictated by mRNA. The main function of EEF1B2 is to encode a subunit of the elongation factor-1 (EF-1) complex. This complex is responsible for the delivery of aminoacyl-tRNA to the ribosome during the elongation phase of translation. The protein product of EEF1B2 is involved in several other cellular processes in addition to its canonical role in translation elongation. For example, it is involved in regulating the cytoskeleton, cell morphology, and the cell's response to external signals and stress.
Abnormalities in EEF1B2 expression or function are associated with various pathologies, including cancer. Overexpression or mutations within EEF1B2 have been observed in certain cancer types, suggesting that it may play a role in tumorigenesis by promoting an increased demand for protein synthesis in rapidly dividing cancer cells or through regulatory pathways that have not yet been fully elucidated.
The EEF1B2 (eukaryotic translation elongation factor 1 beta 2) gene encodes a protein involved in the elongation step of protein synthesis. By establishing a 293T cell line with EEF1B2 knockout, researchers can study various biological processes and disease mechanisms. Here are some applications of the EEF1B2 knockout cell line-293T:
Cancer Research: By understanding the role of EEF1B2 in cell proliferation and survival, researchers can study its mechanism of promoting tumorigenesis and metastasis, which can help develop targeted cancer therapies.
Gene Function Studies: This knockout model helps to characterize its specific functions by observing phenotypic changes after the loss of the EEF1B2 gene, thereby gaining a deeper understanding of its biological role.
Protein Synthesis Insights: Since EEF1B2 is involved in the protein translation process, studying the loss of EEF1B2 in 293T cells can provide insights into the translation mechanism and provide potential targets for diseases related to dysregulated protein synthesis.
Drug Screening: EEF1B2 knockout 293T cells can be used to screen and identify drugs that specifically modulate EEF1B2-dependent pathways, thereby facilitating the discovery of new therapeutic agents.
Disease Modeling: This cell line can mimic diseases in which EEF1B2 plays a key role, providing a valuable in vitro system for studying disease pathogenesis and progression.
Functional Genomics: Genome-wide studies using EEF1B2 knockout cell lines can reveal interaction networks and genetic pathways affected by EEF1B2, enhancing our understanding of cellular function and regulation.
For research use only. Not intended for any clinical use.
