CD38 Knockout Cell Line-HeLa
Cat.No. : CSC-RT2055
Host Cell: HeLa Target Gene: CD38
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
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Cell Line Information
Cell Culture Information
Safety and Packaging
| Cat. No. | CSC-RT2055 |
| Cell Line Information | HeLa -CD38(-/-) is a stable cell line with a homozygous knockout of human CD38 using CRISPR/Cas9. |
| Target Gene | CD38 |
| Host Cell | HeLa |
| Shipping | 1 vial of knockout cell line |
| Storage | Liquid nitrogen |
| Species | Human |
| Gene Symbol | CD38 |
| Gene ID | 952 |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:4-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Background
Applications
CD38, also known as cyclic ADP-ribose hydrolase, is a multifunctional enzyme encoded by the CD38 gene located on human chromosome 4. This gene belongs to the ADP-ribosyl cyclase family and plays a vital role in various physiological and pathological processes. CD38 is widely expressed in a variety of cell types, including immune cells (such as T cells, B cells and natural killer cells) as well as non-immune cells (such as muscle cells, pancreatic β cells and neurons). One of the main functions of CD38 is to participate in calcium signaling. It catalyzes the conversion of NAD+ to cyclic ADP-ribose (cADPR), which is a potent calcium mobilizer. cADPR regulates the release of intracellular calcium stores, thereby participating in important cellular processes such as muscle contraction, insulin secretion and neurotransmitter release.
In the immune system, CD38 plays a variety of roles. As an exoenzyme, it regulates lymphocyte adhesion, migration and signal transduction. Its expression is upregulated upon cell activation and differentiation cues. This makes CD38 a valuable marker for various stages of immune cell differentiation, especially in hematopoietic stem cells and plasma cells. In the clinic, CD38 is used as a target for monoclonal antibody therapy, particularly in the treatment of multiple myeloma, a plasma cell malignancy. Anti-CD38 antibodies, such as daratumumab, have shown effectiveness in eliminating malignant cells by inducing apoptosis, antibody-dependent cellular cytotoxicity (ADCC), and complement-dependent cytotoxicity (CDC).
CD38 is a multifunctional enzyme involved in cell signaling, calcium signaling, and cyclic ADP-ribose metabolism, playing a key role in a variety of physiological and pathological processes. Here are some key applications of the CD38 Knockout Cell Line-HeLa:
Cancer Research: Because CD38 is associated with cell proliferation and survival, depletion of CD38 in the cervical cancer cell line HeLa cells can provide insight into its contribution to tumor growth and resistance to apoptosis.
Immunotherapy Development: CD38 is a target for certain immunotherapies, especially in hematological malignancies. By utilizing CD38 knockout HeLa cells, researchers can test the specificity and efficacy of novel therapeutic antibodies or small molecules against CD38, thereby improving these treatments.
Signal Transduction Studies: CD38 is involved in a variety of signaling pathways, including calcium signaling and NAD+ metabolism. Knockout HeLa cells allow researchers to dissect these pathways in the absence of CD38, helping to identify downstream effects and potential compensatory mechanisms.
Drug Screening: CD38 knockout HeLa cells are a valuable tool for high-throughput drug screening, particularly for compounds designed to modulate CD38 activity.
Metabolic Studies: Given the role of CD38 in NAD+ metabolism, studying knockout HeLa cells can reveal how NAD+ levels and related metabolic processes are altered.
For research use only. Not intended for any clinical use.
