BRD4 Knockout Cell Line-293T

BRD4 Knockout Cell Line-HEK293T

Cat.No. : CSC-RT0467

Host Cell: HEK293T Target Gene: BRD4

Size: 1x10^6 cells/vial, 1 mL Validation: Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No. CSC-RT0467
Cell Line Information This cell line is a stable cell line with a homozygous knockout of human BRD4 gene using CRISPR/Cas9.
Target Gene BRD4
Gene ID 23476
Genotype BRD4 (-/-)
Host Cell HEK293T
Size 1x10^6 cells/vial, 1 mL
Sequencing Result Homozygous: 2 bp deletion in exon1
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium Complete medium supplemented with 10% (v/v) DMSO
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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Background

Applications

BRD4, or bromodomain-containing protein 4, is an important member of the BET (bromodomain and extra-terminal domain) protein family, which includes BRD2, BRD3, and BRDT. These proteins play a crucial role in regulating gene expression by recognizing and binding to acetylated lysine residues on histone tails, a process that is integral to the regulation of chromatin structure and transcriptional machinery. BRD4 is unique in its ability to remain associated with chromatin throughout the cell cycle, even during mitosis. This constant association ensures rapid reactivation of genes after mitosis, helping to maintain cellular identity and function. Functionally, BRD4 is a master regulator of transcription and plays a key role in various cellular processes, including cell cycle progression, apoptosis, and cell differentiation. It is best known for its role in the transcriptional regulation of key oncogenes such as MYC. Therefore, BRD4 has attracted great attention in cancer research, as its dysregulation has been associated with a variety of malignancies, including leukemia, breast cancer, and prostate cancer. In terms of therapy, BRD4 has emerged as a promising target for drug development, leading to the generation of BET inhibitors. These small molecules, such as JQ1 and OTX015, block the interaction between BRD4 and acetylated histones, thereby inhibiting the transcription of growth-promoting genes and exhibiting anticancer properties.
1. Cancer Research: BRD4 is a member of the Bromodomain and Extra-Terminal (BET) family and is known to play a critical role in the regulation of gene expression, particularly in oncogenesis. Using BRD4 knockout 293T cells allows researchers to investigate the specific functions of BRD4 in cancer cell proliferation, apoptosis, and metastasis. 2. Drug Development: BRD4 knockout 293T cells can serve as a control to demonstrate the specificity and efficacy of BRD4 inhibitors. This can expedite the development and testing of new therapeutic compounds aimed at modulating BRD4 activity. 3. Epigenetic Studies: BRD4 is implicated in reading and interpreting epigenetic marks. Therefore, BRD4 knockout cells provide an invaluable tool for studying how the absence of BRD4 affects chromatin structure, histone modifications, and gene expression. 4. Signal Transduction Pathways: Researchers can use BRD4 knockout cells to study its role in various signaling pathways, including those related to cell cycle progression, DNA damage response, and inflammation. 5. Viral Gene Expression: BRD4 is known to interact with viral proteins and can influence the expression of viral genes. Utilizing BRD4 knockout 293T cells can help decipher the role of BRD4 in viral replication and latency, providing insights into viral pathogenesis and potential antiviral strategies. 6. High-Throughput Screening: BRD4 knockout 293T cells can be used in high-throughput screening assays to identify novel genes, pathways, or compounds that can compensate for the loss of BRD4. This can lead to the discovery of new therapeutic targets and biomarkers.

For research use only. Not intended for any clinical use.
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