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As for the study of genome-scale DNA methylation, there are several common methods to be chosen, such as traditional whole-genome bisulfite sequencing. Despite its advantages of high resolution and comprehensive detection for methylation, this method needs relatively great amount of starting material. At least 5μg of genomic DNA is needed for the library construction. So it is inappropriate for the samples with limited starting material.
DNA methylation is a common modified approach discovered early by scientists in the field of molecular biology. A large number of studies have shown that DNA methylation can regulate gene expression by causing changes to chromatin structure, DNA conformation and stability, as well as the interactions between DNA and proteins.
Summary
Lentivirus (Lentivirus) vector is gene therapy vectors, which is based on HIV-1 (human immunodeficiency virus type I). Compared to those general retroviral vectors, it has the ability to infect dividing cells and non-dividing cells. Lentiviral can produce the high-titer lentiviral, which can be used in shRNA expression.
This blog article is to discuss classification of cell transfection technology, as well as introducing the principles, applications and characteristics of different methods, and to compare various of transfection methods.
Lentivirus is classified as retroviridae. The pre-integration complex of the lentivirus nuclear protein has the characteristics of phagocytosis, and the viral genome translocates to the nucleus, so that it can infect and replicate in the non-mitotic cells. This feature makes it an ideal transfer vector for gene therapy.
