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It has been over 30 years since the first generation of DNA sequencing technology was developed in 1977. During this period, sequencing technology has made considerable progress. From the first generation to the third generation and even the fourth generation, sequencing technology has experienced the read length from long to short, and short to long. Although the second generation---short-read sequencing technology still dominates the current global sequencing market, the third and fourth generation of sequencing technologies are rapidly evolving over the course of the two-year period. Every transformation of sequencing technology results in a huge role in promoting genome research, disease medical research, drug development, breeding and other fields. This blog is mainly focusing on the current genome sequencing technologies and their sequencing principles.
As for the study of genome-scale DNA methylation, there are several common methods to be chosen, such as traditional whole-genome bisulfite sequencing. Despite its advantages of high resolution and comprehensive detection for methylation, this method needs relatively great amount of starting material. At least 5μg of genomic DNA is needed for the library construction. So it is inappropriate for the samples with limited starting material.
