Overview

Creative Biogene is dedicated to delivering high-quality, rapid, and cost-effective stable cell line generation services to support protein expression, gene knockdown, and genome editing for specialized research needs. With a wealth of experience, our cell culture scientists focus on fine-tuning every parameter to meet specific requirements, using proprietary expression vectors, advanced high-throughput screening technologies, and serum- and animal-component-free processes to generate reliable and adaptable cell lines in reduced timelines.

Service Highlights

Core Services

Client Case Studies & Research Outcomes

Creative Biogene has collaborated with various biotechnology companies and academic researchers to create reliable, functional cell lines for different applications. Our case studies demonstrate how our customized cell line development has contributed to breakthroughs in areas like cancer research, drug discovery, and gene therapy.

Case Study 1

One of the most aggressive cancers is breast cancer, which can spread to other organs and invade nearby tissues. The researchers developed a 3D culture system using composite gels made of alginate and Matrigel to simulate the tumor microenvironment. Creative Biogene contributed to this study by constructing a GFP-expressing stable cell line (MDA-MB-231) that enabled real-time visualization of cellular behavior within the gel. This GFP stable cell line allowed for continuous tracking of cancer cell migration, intravasation, and invasion, supporting the researchers' innovative 3D model to analyze the early steps of metastasis.

Figure 1. The researchers characterized MDA-MB-231 cell morphology in various gel concentrations, utilizing GFP-expressing stable cells to visualize malignant features. (Cavo M, et al., 2018).

Case Study 2

Polycystic ovarian syndrome (PCOS) is a prevalent endocrine condition that frequently causes estrogen insufficiency and insulin resistance. The researchers aimed to investigate the role of WNT signaling in regulating estrogen synthesis in PCOS. They found that FZD3 expression was significantly increased in cumulus cells (CCs) from PCOS patients. This upregulation, along with activation of the WNT2/β-Catenin pathway, was closely associated with estrogen deficiency. The researchers used FZD3-overexpressing cells from Creative Biogene to study its impact on estrogen production and found that it impaired FSH-induced estrogen synthesis. This study highlights the potential of targeting FZD3 in CCs as a therapeutic strategy for restoring estrogen production in PCOS. Our stable cell line services, like the FZD3-overexpressing system, offer researchers robust tools to study gene function and signaling pathways in various disease models.

Figure 2. The researchers used FSHR-overexpressing HEK293 cells, transfected with FZD3 and related plasmids, to study FZD3's effect on FSH-stimulated steroidogenesis. (Qiao GY, et al., 2017)

Case Study 3

The SARS-CoV-2 epidemic has motivated researchers to look into how the virus infects lung cells, including its interaction with the human ACE2 receptor. The researchers utilized computational approaches to analyze the differences between SARS-CoV and SARS-CoV-2 spike proteins, revealing key alterations that enhance the binding affinity of SARS-CoV-2 to ACE2. To further explore the virus's impact, the researchers employed A549 cells expressing ACE2, which were procured and cultured using Creative Biogene's services.

Figure 3. The researchers conjugated SARS-CoV-2 spike protein on gold nanourchins and compared its effects with SARS spike protein using ACE2/TMPRSS-expressing A549 cells to analyze infectivity through FACS and ICP-MS. (Singh AV, et al., 2022)

Service Support

Customized Construction Methods

At Creative Biogene, we offer customized construction methods to fit a variety of research applications, each paired with an ideal delivery solution. Our technical support team is ready to provide one-on-one consultations to help you select the best approach. Leveraging our expertise in gene editing, we design optimized sgRNA and donor templates for precise gene knock-ins or knockouts at popular safe-harbor sites, including AAVS1 (human) and Rosa26 (mouse).

To meet your specific requirements, we provide flexible Cas9 delivery options such as stable Cas9 cell lines, lentiviral, adenoviral, and Cre-LoxP models. For long-term results, we recommend early cryopreservation of stable clones and regular selective pressure to maintain consistent gene expression. With shRNA lines, note that expression may decline after ten passages, so early planning and support are key. Contact Creative Biogene to explore tailored solutions that drive impactful results in your research.

Quality Control

We maintain stringent quality control throughout the development process:

• Genetic Validation: Sequence verification, copy number analysis, and genomic integration confirmation.
• Expression Validation: Quantification of RNA and protein expression, stability assessments, and functional verification.
• Microbial Testing: All cell lines are mycoplasma-free, and sterility tests are conducted as part of our rigorous validation process.

Contact Us

Creative Biogene is a national high-tech enterprise aiming to lead the global biopharmaceutical services industry. Having worked in the biopharmaceutical business for more than ten years, we provide specialized research platforms, state-of-the-art technology, and skilled project management to assist global industrial and academic development. We prioritize client demands and offer customized services to researchers and biopharmaceutical firms, promoting R&D in the sector.

Use Creative Biogene's skilled cell line creation services to expedite your study. Get in touch with us right now to find out how our tailored solutions can help you with your work.

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