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miRNA sponge functional model plays a very significant role in biological researches. In this blog, we mainly introduce professor Rajewsky Nikolaus, who have contributed a lot for the circular RNA function study.
As for the study of genome-scale DNA methylation, there are several common methods to be chosen, such as traditional whole-genome bisulfite sequencing. Despite its advantages of high resolution and comprehensive detection for methylation, this method needs relatively great amount of starting material. At least 5μg of genomic DNA is needed for the library construction. So it is inappropriate for the samples with limited starting material.
DNA methylation is a common modified approach discovered early by scientists in the field of molecular biology. A large number of studies have shown that DNA methylation can regulate gene expression by causing changes to chromatin structure, DNA conformation and stability, as well as the interactions between DNA and proteins.
Summary
Lentivirus (Lentivirus) vector is gene therapy vectors, which is based on HIV-1 (human immunodeficiency virus type I). Compared to those general retroviral vectors, it has the ability to infect dividing cells and non-dividing cells. Lentiviral can produce the high-titer lentiviral, which can be used in shRNA expression.
