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Experiment Tags: PCR Amplification, Probe Preparation
Experiment Summary: This experiment introduces the PCR labeling technique of probes using biotin and digoxin as examples.
Experiment Tags: Membrane Protein, Bacterial Total Protein, Protein Extract
Experiment Summary: This experiment describes several methods for extracting total bacterial proteins and membrane proteins.
Experiment Tags: Agrobacterium-Mediated, Rice, Gene Transformation
Experiment Summary: This experiment describes Agrobacterium-mediated transformation of rice.
Experiment Tags: Lentivirus, Lentivirus Concentration, Lentivirus Purification
Experiment Summary: This experiment introduced the operation steps of concentrating and purifying lentivirus by ultracentrifugation precipitation method.
Experiment Tags: Lentivirus, Lentivirus Concentration, Lentivirus Purification
Experiment Summary: This experiment introduced the operation steps of concentrating and purifying lentivirus with PEG-8000 concentration method.
Experiment Tags: PCR Amplification, Probe Hybridization, ELISA Assay
Experiment Summary: This experiment describes the procedure of PCR-ELISA experiment based on immunological method to detect PCR products.
Experiment Tags: Cell, Adherent Cells, Suspension Cells
Experiment Summary: If the cell viability is >85% and the generation time is similar to that observed with the original medium, it suggests that the cells adapt to growth in current condition.
Experiment Tags: Cell, Cell Line Generation
Experiment Summary: Since mammalian cells sensitivity to antibiotics varies from one cell type to another, it is essential to determine the minimum concentration of antibiotic required to kill non-transfected or non-transduced cells by separate kill curve experiments.
Experiment Tags: Adenovirus
Experiment Summary: In our protocol, the recombination between the shuttle plasmid and the adenovirus genome contained in the backbone plasmid is performed in the E. coli strain. Positive recombinants are selected by resistance to an antibiotic.
Experiment Tags: Cell, Cell Line Generation, Lentivirus
Experiment Summary: This protocol can be used to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.
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