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Experiment Tags: Flavivirus, Fluorescence, NS2B-NS3 (NS2B-NS3), Live-cell imaging, Reporter cells, Plaque assay, Image analysis
Experiment Summary: This protocol describes methods for generating, selecting and implementing stable BHK-21 cells that express molecular reporters encoding viral genes suitable for monitoring viral infection by live cell imaging.
Experiment Tags: Cell Immortalization, Lentiviral Transduction
Experiment Summary: The protocol introduces the definition of cell immortalization and commonly used viral transfection methods, and describes in detail the steps to generate immortalized cells using prefabricated cell immortalization lentiviral particles.
Experiment Tags: CRISPR, Cpf1, AAV, CAR-T, T Cell, Cancer, Immunotherapy
Experiment Summary: This protocol describes a new method, the AAV-Cpf1 KIKO system, using a combination of viral and non-viral approaches to generate a stable CAR-T with homology-directed repair (HDR) knock-in and immune checkpoint knockout at high efficiency in one step.
Experiment Tags: NLR, NOD1, Lentivirus, Stable Expression, TRE, Tetracycline
Experiment Summary: In this protocol, we describe a method for stable, conditional expression of Nod-Like receptors (NLRs) in THP-1 cells using a lentiviral expression system.
Experiment Tags: Lentivirus, Tumor, Lymphoma, Luciferase, GFP, Mouse
Experiment Summary: In this protocol, we describe methods for the production of stable luciferase expressing tumor cell lines by lentiviral transduction.
Experiment Tags: Plasmid DNA, Transformation, Drug-Resistant Plasmids
Experiment Summary: This experiment describes the method of transferring plasmid DNA molecules into E. coli and how to screen cell clones with plasmid DNA, i.e. transformants.
Experiment Tags: Genomic, Overexpression, Library
Experiment Summary: This protocol might work for searching complementation of other gene deletion mutants that have a good selective phenotype.
Experiment Tags: Particle bombardment, Maize embryos, Promoter expression, Quantitative analysis
Experiment Summary: This protocol describes the entire process of transient transformation of maize embryos using particle bombardment.
Experiment Tags: Ex vivo tissue culture model, Lentiviral, Proliferation, Oncogenic , Transformation, Prostate
Experiment Summary: This protocol describes how to generate lentiviral vectors and particules, successfully transduce human prostate tissues, induce exogenous gene expression, and assess cellular proliferation.
Experiment Tags: Lentivirus, Stable cell lines, Gene knockdown, Gene over-expression, Mammalian cell lines, Transduction
Experiment Summary: This protocol describes the use of the 3rd generation lentiviral system which uses three different plasmids for generating stable cell lines.
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