Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Experiment Tags: CircRNAs, RNA Pull-Down, RNA-Protein Interactions
Experiment Summary: In this protocol, we introduce a method to study RNA-protein interactions, which will facilitate researchers to carry out circRNA related experiments.
Experiment Tags: RSV Transcription, Minigenome, Polymerase, Luciferase Assay, Real-Time Quantitative PCR Assay
Experiment Summary: In this protocol, we detail two different methods of detecting viral RNA synthesis: a firefly luciferase assay for rapid and sensitive measurement of RSV polymerase activity; and a real-time quantitative PCR method for determination of specific effects on the transcription of individual viral genes and the polar transcription gradient of RSV.
Experiment Tags: CRISPRCas9 Library Screening, CRISPRCas9
Experiment Summary: In this protocol, we briefly describe the CRISPR/Cas9 library screening process to simplify and facilitate high-throughput screening.
Experiment Tags: TAC-Seq, Chromatin Accessibility, Genome Wide, Zebrafish, Tn5 Transposase
Experiment Summary: In this article, we describe the application of ATAC-seq in zebrafish embryos, thus illustrating the applicability of this method to the entire vertebrate population.
Experiment Tags: Adeno-Associated Virus, Binding, Internalization, Virus Entry, Virus, Cell Surface
Experiment Summary: In this protocol, we describe a quantitative PCR (qPCR) based method to determine the amount of vector bound to the cell surface and the amount of subsequent virus internalization based on viral genome quantification.
Experiment Tags: Circular RNA, In Vitro Transcription, Ligase, Phosphorylation, Polyacrylamide Gel Electrophoresis
Experiment Summary: This protocol describes a simple and straightforward way to obtain single-stranded circular RNA sequences in vitro.
Experiment Tags: CircRNAs, CircRNA Pulldown, Antisense Oligo, Biotin Pulldown, RT-qPCR, miRNA, RNA-Binding Proteins
Experiment Summary: In this detailed protocol, the circRNA of interest (e.g., circHipk2) was captured using a biotin-labeled antisense oligo (ASO) targeting the circHipk2 backsplice junction sequence followed by pulldown with streptavidin-conjugated magnetic beads. The specific enrichment of circRNA was analyzed using reverse transcription quantitative PCR (RT-qPCR).
Experiment Tags: MicroRNA, 3'UTR (3’UTR), Luciferase Assay, In Vitro
Experiment Summary: This protocol is to be used to test the binding and activity of miRNA on putative UTR target sequences. It is based on the expression of Luciferase as a reporter gene fused to the UTR sequence in the presence of plasmids containing pre-miRNA of interest or synthetic miRNA to test in an in vitro cell culture assay.
Experiment Tags: Glycine Oxidase Mutant Libraries, Random Mutagenesis
Experiment Summary: This protocol describes the construction of a glycine oxidase mutant library by random mutagenesis and the use of a phage T7 lysis-based approach for high-throughput screening of oxidase activity, among other things.
Experiment Tags: Long Noncoding RNAs, RNA-Protein Interaction, Mass Spectrometry
Experiment Summary: This article provides a detailed protocol for the assay with hands-on tips for the LnRNA pulldown assay, which is commonly used to identify protein partners of the cellular environment that interact with InRNA.
Copyright © Creative Biogene. All rights reserved.