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Experiment Tags: Adenovirus, Recombinant Adenovirus
Experiment Summary: Conventional approaches to producing small volumes of viral vectors involve culturing cells in stationary and adherent cultures.
Experiment Tags: Lipopolysaccharide, LPS Extraction
Experiment Summary: Here, we describe a hot aqueous-phenol method for the isolation and purification of LPS from gram-negative bacterial cells.
Experiment Tags: BCA Library, Library, Library Construction
Experiment Summary: The size-fractionated DNA is cloned into a BAC vector and transformed into E. coli by electrical shock. The transformants are arrayed into microtiter dishes and high-density replica filters are prepared to facilitate screening of the library.
Experiment Tags: cDNA Library, Library, Library Construction
Experiment Summary: To create a cDNA library, these mRNA molecules are treated with the enzyme reverse transcriptase, which is used to make a DNA copy of an mRNA (i.e., cDNA).
Experiment Tags: Viral Titering, TCID50 Assay
Experiment Summary: A TCID50 was found of greatest value when lack of experimental material or costs of analysis precludes extensive replicate titer determinations but good estimates of titers and treatment differences are essential.
Experiment Tags: Viral Titering, Plaque Assay
Experiment Summary: After fixing and staining the infected cellular monolayer, plaques are counted so that titer viral stock samples in terms of plaque forming units (pfu) per milliliter.
Experiment Tags: Cell, Transfection, Transient transfection
Experiment Summary: Transient transfection means that the constructed plasmids are introduced into mammalian cells in some ways, but the foreign genes of the plasmid are not integrated into the genome of the mammalian cell.
Experiment Tags: T7E1, T7E1 Assay
Experiment Summary: Direct PCR product could be used for T7E1 detection.
Experiment Tags: PCR, Standard PCR
Experiment Summary: Taq polymerase is frequently used in the polymerase chain reaction (PCR), for it is able to withstand repeated heating to 95 °C without significant loss of activity, an efficient method for greatly amplifying the quantity of DNA templates.
Experiment Tags: RT-PCR, PCR
Experiment Summary: RNA is first reverse transcribed into cDNA using a reverse transcriptase as described here, the resulting cDNA is used as templates for subsequent PCR amplification using primers specific for one or more genes.
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