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Protocols

protocols
Protocol

Protocol for Detection and Analysis of Cyclic RNA Using RT-PCR

Experiment Tags: Circular RNA, Backsplice junction, Divergent primer, RT-PCR (RT-PCR), RNase R (RNase R)

Experiment Summary: This protocol describes the design of divergent primers which face away from each other on the linear RNA, so that they can only amplify the circRNAs, and not the linear RNAs with the same sequence.

protocols
Protocol

Production of Neuron-Preferential Lentiviral Vectors Protocol

Experiment Tags: Neuron-preferential, Lentiviral vectors

Experiment Summary: This protocol describes the use of the 3rd generation lentiviral system which uses three different plasmids for generating stable cell lines.

protocols
Protocol

Transformation of Arabidopsis Thaliana Protocol

Experiment Tags: Arabidopsis Thaliana, Transgenic

Experiment Summary: This experiment describes the use of the flower immersion method to transfer target genes into Arabidopsis thaliana using Agrobacterium mediated transfer.

protocols
Protocol

Labeling Protocol for Microarray Analysis

Experiment Tags: Labeling,Microarray Analysis

Experiment Summary: This experiment describes how to perform labeling procedure for microarray analysis.

protocols
Protocol

Construction of Transgenic Zebrafish Protocol

Experiment Tags: Green Fluorescent Protein, Zebrafish, Transgenic

Experiment Summary: In this experiment, EGFP-containing plasmid was introduced into zebrafish to observe its expression in animals. In zebrafish, green fluorescent protein could be observed under fluorescence microscope from proto-intestinal embryo to emergence stage.

protocols
Protocol

Construction of Plant Expression Vector

Experiment Tags: Agrobacterium-mediated, Vector Construction, Plant Expression Vector

Experiment Summary: This experiment introduces a method for constructing plant expression vectors.

protocols
Protocol

Virus Cryopreservation Technology

Experiment Tags: Virus, Cryopreservation

Experiment Summary: This experiment describes the method for rapid cryopreservation of viruses.

protocols
Protocol

Transmembrane of RNA Samples (Siphon Blotting Method)

Experiment Tags: Transmembrane, Siphon Blotting, RNA Samples

Experiment Summary: This experiment introduces the procedure of transmembrane (i.e., siphon blotting) of RNA samples, etc.

protocols
Protocol

Titration of Virus Infectivity

Experiment Tags: Virus, Titration, Infectivity, TCID5

Experiment Summary: This experiment presents a method for the titration of the infectivity of two viruses.

protocols
Protocol

Assay of Cell Apoptosis (Annexin-V-FITC)

Experiment Tags: Detection, Apoptosis, Annexin-V-FITC, Cells

Experiment Summary: This experiment introduces the principle and procedure of the assay of cell apoptosis (Annexin-V-FITC).

* For research use only. Not intended for any clinical use.
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