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Experiment Tags: Glioma, Retrovirus, Intracerebral Injection, Murine Model, PDGFBB(PDGFBB), PTEN(PTEN), P53(P53)
Experiment Summary: This protocol describes a simple and practical method for inducing GBM formation by retrovirus injection in the murine brain. This system gives a spatial and temporal control over the induction of glioma and allows the assessment of therapeutic effects with a bioluminescent reporter.
Experiment Tags: Base-Editing, CRISPR-Cas9
Experiment Summary: This article provides optimized protocols (including experimental design, methods, and analyses) for designing base editing constructs for basic research.
Experiment Tags: Adeno-Associated Virus (AAV), PEG Aqua Partitioning, AAV Viral Purification, Pancreatic Ductal Infusion, Pancreatic Cell Lineage Tracing
Experiment Summary: This protocol describes efficient delivery of AAV to mediate GFP or Cre expression for cell lineage tracing in the mouse pancreas or for the delivery of transgenes under a specific promoter to these cells.
Experiment Tags: Single Cell, Genomics, Molecular Biology, Gene Expression, CRISPR
Experiment Summary: This protocol describes the detailed steps for generating knockout cell lines (coding or non-coding regions) using CRISPR/Cas9 tools.
Experiment Tags: Inflammation, Peritonitis, Sepsis, In Vivo Imaging, Myeloperoxidase
Experiment Summary: This protocol allows studying of inflammatory response induced by LPS that affect neutrophil infiltration by tracking myeloperoxidase (MPO) activity in vivo.
Experiment Tags: Gene Transfer, Central Nervous System (CNS), Adenoviral Vectors
Experiment Summary: This protocol describes a procedure for the direct injection of adenovirus into the CNS using stereotaxic guidance.
Experiment Tags: Cytotrap System, Yeast Two-Hybrid Screen, Protein Interaction
Experiment Summary: This protocol describes the construction of a cDNA library from the model plant Arabidopsis and a procedure for screening interaction proteins of AtSR1/CAMTA3, a Ca2+/CaM-regulated transcription factor from this library.
Experiment Tags: Retroviral Vectors, Purification, Injection.
Experiment Summary: This protocol discusses the production, purification, and use of replication-deficient retroviral vectors based on Moloney murine leukemia virus and lentivirus. It also describes the injection of a retroviral vector into the dentate gyrus of young adult mice to fluorescently label live murine brain tissue.
Experiment Tags: Lentiviral Transduction, Mouse Induced Pluripotent Stem Cells.
Experiment Summary: This protocol describes a method for generating and characterizing iPS cells from mouse embryonic fibroblasts using a lentiviral transduction system.
Experiment Tags: AAV Vectors, Intracranial Stereotactic Injection, AAV Delivery
Experiment Summary: This protocol describes how to perform an aseptic stereotaxic survival surgery to deliver AAV vectors to the brain of a mouse.
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