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Experiment Tags: RNAi
Experiment Summary: RNAi consists of inititation and effector steps. At inititation steps, the dsRNAs get processed into 21-23 nucleotide (nt) small interfering RNAs (siRNAs) by an RNase III-like enzyme called Dicer.
Experiment Tags: Lentivirus, Recombinant Lentivirus
Experiment Summary: Transient transfection method is now widely used for generation of recombinant lentivirus. Envelope plasmid, packaging plasmid and recombinant lentivirus plasmid cotransfect 293T cells to get producing cells.
Experiment Tags: PERT Assay, Product-enhanced RT
Experiment Summary: Sensitivity was greatly increased when a PCR amplification step of the synthesized cDNA was introduced prior to product detection. These types of assays are known as product-enhanced RT (PERT) assays.
Experiment Tags: Dilution, Cell
Experiment Summary: Limiting dilution analysis attempts to determine the frequency of cells having a particular function that are present in a mixed population of cells. So limiting dilution analysis is an easy-operated method that used to measure the abundance of cells able to perform a particular function.
Experiment Tags: Lentivirus, Lentivirus Transduction
Experiment Summary: Lentivirus is a modified HIV virus that must be handled with caution although it cannot replicate in a host.
Experiment Tags: sgRNA, CRISPR
Experiment Summary: Selection of sgRNAs for an experiment needs to balance two aspects: maximizing on-target activity while minimizing off-target activity, which sounds obviously but can often require thorough decisions.
Experiment Tags: Cell, Cell Line
Experiment Summary: The fundamental principle is to add regulatory elements to the upstream of the target gene through artificial construction, so that genes can be transcribed and translated efficiently under controlled conditions.
Experiment Tags: Fosmid Library, Library, Library Construction
Experiment Summary: The fosmid system has been widely used in the construction of large-insert genomic libraries. Fosmid clones are similar in insert size comparing with cosmid clones and smaller than that in BAC and yeast artificial chromosomes (YAC).
Experiment Tags: CRISPR, CRISPR Plasmid
Experiment Summary: The CRISPR/Cas9 system has two critical parts: sgRNA sequence and Cas9 sequence. These two sequences could be cloned into separated plasmids or combined into one plasmid, each format has its own set of advantages and shares a similar delivery procedure.
Experiment Tags: Codon Optimization, Codon
Experiment Summary: Codon optimization is one of the key steps in achieving the high level expression of target gene. There are some key factors for consideration including transcription efficiency, translation efficiency, gene synthesis and protein folding.
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